聚苯乙烯微/纳塑料暴露对小鼠RAW264.7巨噬细胞mRNA表达谱的影响  

Exposure of polystyrene micro/nanoplastics on the mRNA expression profile of murine RAW264.7 macrophages

作　　者：任肖敏 杨忠能 王小霞 何欢[1] 胥志祥 潘学军[1] REN Xiaomin;YANG Zhongneng;WANG Xiaoxia;HE Huan;XU Zhixiang;PAN Xuejun(Faculty of Environmental Science and Engineering,Kunming University of Science and Technology,Kunming 650500;College of Resources and Environmental Engineering,Tianshui Normal University,Tianshui 741000)

机构地区：[1]昆明理工大学环境科学与工程学院,昆明650500 [2]天水师范学院资源与环境工程学院,天水741000

出　　处：《环境科学学报》2024年第11期370-377,共8页Acta Scientiae Circumstantiae

基　　金：国家自然科学基金项目(No.22276082);云南省重大科技项目(No.202302AG050001);云南省科技计划项目(No.202101BE070001-002);昆明理工大学“双一流”创建医学联合专项面上项目(No.KUST-AN2023004Y);天水师范学院校列创新基金(NO.CXJ2023-03)。

摘　　要：当前,微/纳塑料对人类和动物的潜在健康危害效应受到了广泛关注.本文基于基因组学分析方法,以3μm和75 nm的聚苯乙烯塑料微球作为微塑料和纳塑料的模型,以小鼠RAW264.7巨噬细胞作为细胞模型,研究了微/纳塑料对该细胞基因表达的影响,以探讨微/纳塑料对巨噬细胞免疫功能的潜在影响.结果表明,纳塑料导致RAW264.7细胞差异表达基因数量(1μg·mL^(-1)暴露浓度,143个)要多于微塑料(1μg·mL^(-1)暴露浓度,82个);通过GO(Gene Ontology)对差异表达基因分析发现,微/纳塑料均能影响RAW264.7细胞的生物学过程、分子功能、细胞组成这三大生物学功能.通过KEGG(Kyoto Encyclopedia of Genes and Genomes)分析差异基因富集的通路,发现微/纳塑料暴露能影响RAW264.7细胞的免疫/炎症疾病相关通路,如缺氧诱导因子-1(HIF-1)通路和甲型流感(Influenza A)通路等.使用实时荧光定量PCR(qRT-PCR)对10个显著差异表达基因进行验证,实验结果与基因组测序结果一致.综上所述,微/纳塑料暴露可能会导致巨噬细胞产生炎症反应,进而导致机体免疫功能紊乱.The adverse effects of micro/nanoplastics on humans and animals have garnered significant attention.This study utilized genomic analysis methods,employing 3μm and 75 nm polystyrene plastic microspheres as models for microplastics and nanoplastics,respectively,with mouse RAW264.7 macrophage cells as the cellular model.The research focused on assessing the impact of micro/nanoplastics on gene expression in these cells to investigate their potential effects on macrophage immune function.Our findings revealed that nanoplastic exposure led to a greater number of differentially expressed genes in RAW264.7 cells(143 genes at 1μg·mL^(-1))compared to microplastic exposure(82 genes at 1μg·mL^(-1)).Gene Ontology(GO)analysis demonstrated that both microplastics and nanoplastics can influence the biological processes,molecular functions,and cellular components of RAW264.7 cells.Pathway enrichment analysis using KEGG revealed that exposure to micro/nanoplastics can influence immune/inflammatory disease-related pathways in RAW264.7 cells,such as activating the Hypoxia-inducible factor-1(HIF-1)and Influenza A pathways.In conclusion,exposure to micro/nanoplastics may trigger macrophage-mediated inflammatory responses,potentially disrupting immune function in organisms.

关 键 词：微/纳塑料 基因组学 小鼠巨噬细胞 炎症反应 免疫功能 

分 类 号：X171.5[环境科学与工程—环境科学]