水稻籽粒VB6含量的调控位点挖掘及分析  

Mining and analysis of regulatory loci for VB6 content in rice grains

作　　者：唐璐瑶 连锦瑾 赵蓓蓓 沈芷琦 朱哲楠 叶语涵 张芷宁 路梅[1] 王跃星 饶玉春[1] TANG Luyao;LIAN Jinjin;ZHAO Beibei;SHEN Zhiqi;ZHU Zhenan;YE Yuhan;ZHANG Zhining;LU Mei;WANG Yuexing;RAO Yuchun(College of Life Sciences,Zhejiang Normal University,Jinhua 321004,Zhejiang,China;State Key Laboratory of Rice Biology,China National Rice Research Institute,Hangzhou 310006,Zhejiang,China)

机构地区：[1]浙江师范大学生命科学学院,浙江金华321004 [2]中国水稻研究所水稻生物学国家重点实验室,浙江杭州310006

出　　处：《生物工程学报》2024年第10期3485-3499,共15页Chinese Journal of Biotechnology

基　　金：浙江省自然科学基金重点项目(LZ23C130003);2023年度金华市科技局公益性技术应用研究项目(2023-4-059);浙江省大学生科技创新活动计划暨新苗人才计划(2024R404A037)。

摘　　要：维生素B6(vitamin B6,VB6)作为参与动植物多项生命活动的重要组成成分,体现了水稻等粮食经济作物的营养品质。目前对于控制水稻籽粒VB6含量的相关基因的挖掘较少且不深入。本研究以‘华占(HZ)’和‘热研2号(Nekken2)’为亲本所构建的重组自交系为材料,在数量性状基因座(quantitative trait loci,QTL)定位的基础上进行候选基因初步筛选,共获得10个候选基因,其中LOC_Os01g52450、LOC_Os01g52500、LOC_Os05g09500、LOC_Os05g09440、LOC_Os05g20570和LOC_Os05g36270基因的表达量在双亲间差异显著。结合基因表达量和亲本VB6含量数据,推测LOC_Os05g09500是影响水稻籽粒VB6含量的关键基因,其高表达显著影响水稻籽粒VB6含量。本研究填补了水稻籽粒VB6性状QTL定位研究的空白,为进一步阐明水稻VB6合成的分子遗传机理、克隆相关基因提供了理论支持,在鉴定、筛选和培育高VB6含量的水稻新品种方面具有一定意义。Vitamin B6(VB6),as an essential component involved in numerous biological activities of animals and plants,reflects the nutritional quality of cereal crops such as rice.Few studies have been conducted to mine the genes controlling the VB6 content in rice grains,and the available studies remain to be deepened.In this study,the recombinant inbred lines created from parents‘HZ’and‘Nekken2’served as the experimental materials.Based on QTL mapping,the initial screening identified ten candidate genes.The expression levels of LOC_Os01g52450,LOC_Os01g52500,LOC_Os05g09500,LOC_Os05g09440,LOC_Os05g20570,and LOC_Os05g36270 showed significant differences between the parents.According to the gene expression and parental VB6 content,we hypothesized LOC_Os05g09500 as the key gene affecting the VB6 content in rice grains,and the high expression of this gene significantly influenced the VB6 content.The results of this study fill a gap in the QTL mapping on the VB6 content of rice grains and provide theoretical support for elucidating the molecular genetic mechanisms and cloning the related genes of VB6 synthesis in rice.In addition,the findings have significant implications for identifying,screening,and breeding new rice cultivars with high VB6 content.

关 键 词：水稻 维生素B6 数量性状基因座定位 营养品质 

分 类 号：S511[农业科学—作物学]