宫颈癌组织中CSRP2BP mRNA,ESRRB mRNA和蛋白水平表达与上皮间质转化及临床预后相关性研究  

Expression of CSRP2BP mRNA,ESRRB mRNA and Protein Levels in Cervical Cancer Tissue and Their Correlation with Epithelial Mesenchymal Transition and Clinical Prognosis

作　　者：赵建芳 石芳 张昕[1] 贺薇冉 ZHAO Jianfang;SHI Fang;ZHANG Xin;HE Weiran(Department of Pathology,Yan’an Traditional Chinese Medicine Hospital/Yan’an Branch of Peking University Third Hospital,Shaanxi Yan’an 716099,China)

机构地区：[1]延安市中医医院/北京大学第三医院延安分院病理科,陕西延安716099

出　　处：《现代检验医学杂志》2024年第6期96-101,107,共7页Journal of Modern Laboratory Medicine

基　　金：陕西省卫生健康科研项目(2022D0024)。

摘　　要：目的研究宫颈癌组织中富含半胱氨酸蛋白2结合蛋白(cysteine rich protein 2 binding protein,CSRP2BP)mRNA,雌激素相关受体β(estrogen related receptorsβ,ERRB)mRNA和蛋白表达与上皮间质转化(epithelial mesenchymal transition,EMT)及临床预后的相关性。方法选择2018年3月~2020年3月延安市中医医院收治的106例宫颈癌患者。采用实时荧光定量PCR(RT-qPCR)检测组织中CSRP2BP mRNA,ESRRB mRNA及EMT相关指标[E-钙黏蛋白(E-cadherin,E-cad),N-钙黏蛋白(N-cadherin,N-cad),锌指转录因子(Snail family transcriptional repressor,Snail)]表达。采用免疫组织化学检测组织中CSRP2BP蛋白,ESRRB蛋白表达。Pearson相关分析CSRP2BP mRNA,ESRRB mRNA与EMT相关指标的相关性。采用Kaplan-Meier曲线和COX回归分析CSRP2BP mRNA,ESRRB mRNA表达对宫颈癌患者预后的影响。结果癌组织中CSRP2BP mRNA(3.14±0.52 vs 1.22±0.21)和蛋白阳性率(86.79%vs 9.43%),ESRRB mRNA(2.86±0.31 vs 1.06±0.20)和蛋白阳性率(92.45%vs 8.49%),N-cad mRNA(3.22±0.43vs 1.05±0.26),Snail mRNA(2.67±0.36 vs 0.69±0.17)表达高于癌旁组织,E-cad mRNA(0.84±0.17 vs 2.15±0.24)表达低于癌旁组织,差异具有统计学意义(t/χ^(2)=34.249,127.049;50.234,149.466;44.461,51.204,45.858,均P<0.001)。宫颈癌中CSRP2BP mRNA,ESRRB mRNA与N-cad mRNA,Snail mRNA呈正相关(r=0.663,0.731;0.726,0.715,均P<0.001),与E-cad mRNA呈负相关(r=-0.594,-0.669,均P<0.001)。FIGO分期ⅠB2~ⅡA,低分化程度及淋巴结转移宫颈癌患者癌组织中CSRP2BP mRNA(4.24±0.57,4.27±0.58,4.24±0.50),ESRRB mRNA(4.48±0.36,4.21±0.37,4.69±0.33)高于FIGO分期ⅠA~ⅠB1(2.60±0.44,2.06±0.24),中高分化程度(2.43±0.44,2.01±0.25)及无淋巴结转移(2.53±0.58,2.07±0.26)的患者,差异具有统计学意义(t=16.327,41.135;18.507,36.545;14.501,43.806,均P<0.001)。CSRP2BP mRNA高表达三年总生存率为66.00%(33/50),低于低表达组的89.29%(50/56);ESRRB mRNA高表达组三年总生存率为65.38%(34/52),低于低表达组的90.74%(49/54),差异具Objective To investigate the expression of cysteine rich protein 2 binding protein(CSRP2BP)mRNA and estrogen related receptorsβ(ESRRB)mRNA and protein in cervical cancer tissues and their correlation with epithelial mesenchymal transition(EMT)and clinical prognosis.Methods A total of 106 cervical cancer patients admitted to Yan’an Traditional Chinese Medicine Hospital from March 2018 to March 2020 were selected.Real timefluorescence quantitative PCR(RT-qPCR)was used to detect CSRP2BP mRNA,ESRRB mRNA and EMT related indicators[E-cadherin(E-cad),N-cadherin(N-cad),snail family transcriptional repressor 1(Snail)].Immunohistochemistry was used to detect the CSRP2BP protein and ESRRB protein expression in tissues.Pearson correlation analysis was used to analyze the correlation between CSRP2BP mRNA,ESRRB mRNA and EMT related indicators.Kaplan-Meier curve and COX regression were used to analyze the effect of CSRP2BP mRNA and ESRRB mRNA expression on the prognosis of cervical cancer patients.Results CSRP2BP mRNA(3.14±0.52 vs 1.22±0.21)expression and protein positive rate(86.79%vs 9.43%),ESRRB mRNA expression(2.86±0.31 vs 1.06±0.20)and protein positive rate(92.45%vs 8.49%),N-cad mRNA(3.22±0.43 vs 1.05±0.26)and Snail mRNA(2.67±0.36 vs 0.69±0.17)expression in cancer tissues were higher than those in adjacent tissues,while E-cad mRNA(0.84±0.17 vs 2.15±0.24)expression was lower than that in adjacent tissues,and the differences were statistically significant(t/χ^(2)=34.249,127.049;50.234,149.466;44.461,51.204,45.858,all P<0.001).The expression of CSRP2BP mRNA and ESRRB mRNA in cervical cancer was positively correlated with N-cad mRNA and Snail mRNA(r=0.663,0.731;0.726,0.715,all P<0.001),and was negatively correlated with E-cad mRNA(r=-0.594,-0.669,all P<0.001).The expression of CSRP2BP mRNA(4.48±0.36,4.21±0.37,4.69±0.33)and ESRRB mRNA(4.48±0.36,4.21±0.37,4.69±0.33)in cervical cancer tissues of patients with FIGO stageⅠB2~ⅡA,poor differentiation and lymph node metastasis was higher than that of patients

关 键 词：宫颈癌 富含半胱氨酸的蛋白质2 雌激素相关受体β 上皮间质转化 

分 类 号：R737.33[医药卫生—肿瘤] R730.43[医药卫生—临床医学]