脑小血管病患者血清lncRNA BIRF,lncRNA FAL1表达水平与脑白质病变程度的相关性分析  

Correlation Analysis between Serum lncRNA BIRF and lncRNA FAL1 Levels Expression and Degree of White Matter Lesions in Patients with Cerebral Small Vessel Disease

作　　者：张晓璇[1] 魏依兰 于宁 韩玥莹 姚雪 刘瑶 窦志杰[1] ZHANG Xiaoxuan;WEI Yilan;YU Ning;HAN Yueying;YAO Xue;LIU Yao;DOU Zhijie(Department of Neurology,Affiliated Hospital of Chengde Medical University,Hebei Chengde 067000,China)

机构地区：[1]承德医学院附属医院神经内科,河北承德067000

出　　处：《现代检验医学杂志》2024年第6期102-107,共6页Journal of Modern Laboratory Medicine

基　　金：承德市应用技术研究与开发暨可持续发展议程创新示范区专项科技计划项目(202205B075)。

摘　　要：目的探究脑小血管病(CSVD)患者血清长链非编码RNA(lncRNA)脑缺血相关因子(BIRF)、1号染色体上的局部扩增lncRNA(lncRNA FAL1)表达与脑白质病变(WML)程度的相关性分析。方法选取承德医学院附属医院2021年6月~2023年6月收治的102例CSVD患者,根据WML诊断标准将CSVD患者分为WML组(n=72)和非WML组(n=30)。并根据Fazekas评分进一步将WML组分为轻度WML组(n=24)、中度WML组(n=36)和重度WML组(n=12)。采用实时荧光定量聚合酶链式反应(RT-qPCR)检测血清中lncRNA BIRF,lncRNA FAL1水平;采用Pearson相关分析血清lncRNA BIRF,lncRNA FAL1水平的相关性。采用受试者工作特征(ROC)曲线分析血清lncRNA BIRF,lncRNA FAL1水平对CSVD患者发生重度WML的诊断价值。结果WML组患者年龄(70.50±5.86岁)、高血压史(有/无:43/29例)、糖尿病史(有/无:45/27例)、IL-33(68.35±6.80 pg/ml),IL-18(97.78±9.65 ng/L)、泛素羧基末端水解酶L1(UCH-L1)(0.29±0.10μg/L),lncRNA BIRF水平(2.45±0.30)显著高于非WML组(67.10±5.76岁,11/19例,9/21例,62.48±6.13 pg/ml,92.56±9.37 ng/L,0.24±0.06μg/L,1.02±0.11),血清lncRNA FAL1表达(0.52±0.10)显著低于非WML组(1.04±0.15),差异具有统计学意义(t=2.683,4.518,8.978,4.085,2.510,2.550,25.346,20.500,均P<0.05)。轻度WML组、中度WML组、重度WML组CSVD患者血清lncRNA BIRF水平(2.23±0.23,2.47±0.31,2.82±0.42)依次升高,血清lncRNA FAL1水平(0.60±0.15,0.51±0.09,0.40±0.04)依次降低,差异具有统计学意义(F=14.913,13.899,均P<0.05)。Pearson相关分析,WML组患者血清lncRNA BIRF与lncRNA FAL1水平呈负相关(r=-0.603,P<0.001);WML患者血清lncRNA BIRF与Fazekas评分呈正相关(r=0.483,P<0.001),血清lncRNA FAL1与Fazekas评分呈负相关(r=-0.507,P<0.001)。血清lncRNA BIRF,lncRNA FAL1水平单独及二者联合诊断CSVD患者发生重度WML的AUC(95%CI)分别为0.756(0.641~0.850),0.839(0.733~0.915)和0.892(0.796~0.953),二者联合检测优于血清lncRNA BIRF单独检测(Z=2.111,P=0.035)。结论CSVD伴WML患�Objective To explore the correlation between the expression of long non-coding RNA(lncRNA)brain ischemia-related factor(BIRF)and focally amplified lncRNA on chromosome 1(lncRNA FAL1)in serum and the degree of white matter lesions(WML)in patients with cerebral small vessel disease(CSVD).Methods From June 2021 to June 2023,102 CSVD patients admitted to Affiliated Hospital of Chengde Medical University were collected,and these patients were grouped into WML group(n=72)and non WML group(n=30)based on WML diagnostic criteria.According to the Fazekas score,the WML group was further grouped into mild WML group(n=24),moderate WML group(n=36)and severe WML group(n=12).Real-time fluorescence quantitative polymerase chain reaction(qRT-PCR)was applied to detect the levels of lncRNA BIRF and lncRNA FAL1 in serum.Pearson correlation was applied to analyze the correlation between serum lncRNA BIRF and lncRNA FAL1 levels.Receiver operating characteristic(ROC)curve was applied to analyze the diagnostic value of serum lncRNA BIRF and lncRNA FAL1 levels for severe WML in CSVD patients.Results The age(70.50±5.86 years),history of hypertension(Yes/No,43/29),history of diabetes(Yes/No,45/27),IL-33(68.35±6.80 pg/ml),IL-18(97.78±9.65 ng/L),ubiquitin carboxyl terminal hydrolase L1(UCH-L1)(0.29±0.10μg/L)and lncRNA BIRF level(2.45±0.30)of patients in the WML group were higher than those in the non WML group(67.10±5.76 years,11/19,9/21,62.48±6.13 pg/ml,92.56±9.37 ng/L,0.24±0.06μg/L,1.02±0.11),while the expression of serum lncRNA FAL1(0.52±0.10)was lower than that in the non WML group(1.04±0.15),with significant differences(t=2.683,4.518,8.978,4.085,2.510,2.550,25.346,20.500,all P<0.05).The serum lncRNA BIRF levels of CSVD patients in the mild,moderate and severe WML groups(2.23±0.23,2.47±0.31,2.82±0.42)were increased sequentially,while the expression of serum lncRNA FAL1(0.60±0.15,0.51±0.09,0.40±0.04)was decreased sequentially,with significant differences(F=14.913,13.899,all P<0.05).Pearson correlation analysis,the serum

关 键 词：脑小血管病 长链非编码RNA脑缺血相关因子 1号染色体上的局部扩增lncRNA 脑白质病变 

分 类 号：R743.9[医药卫生—神经病学与精神病学] R392.11[医药卫生—临床医学]