HPV阳性宫颈癌组织中AIB1 mRNA和ANCO1 mRNA表达及临床意义  

Expression and Clinical Significance of AIB1 mRNA and ANCO1 mRNA in HPV Positive Cervical Cancer Tissue

作　　者：胥康妮 余艳 张琱[1] 王珂 程琪惠 XU Kangni;YU Yan;ZHANG Diao;WANG Ke;CHENG Qihui(Deprtment of Obstetrics and Gynaecology,the Third People’s Hospital of Mianyang,Sichuan Mianyang 621000,China)

机构地区：[1]绵阳市第三人民医院妇产科,四川绵阳621000

出　　处：《现代检验医学杂志》2024年第6期113-118,共6页Journal of Modern Laboratory Medicine

基　　金：四川省医学科研课题(编号:S20077)。

摘　　要：目的研究人乳头状瘤病毒(HPV)阳性宫颈癌组织中乳腺癌扩增基因1(AIB1)、锚蛋白重复结构域11(ANKRD11/ANCO1)表达与临床病理特征的关系及对术后复发的预测价值。方法选取2018年1月~2020年1月就诊于绵阳市第三人民医院的94例HPV阳性宫颈癌患者癌组织和癌旁组织,以同期50例HPV阴性宫颈癌组织为对照。采用实时荧光定量PCR(RT qPCR)检测HPV阳性宫颈癌癌组织和癌旁组织、HPV阴性宫颈癌组织中AIB1 mRNA,ANCO1 mRNA表达。采用Pearson分析HPV阳性宫颈癌癌组织中AIB1 mRNA与ANCO1 mRNA表达的相关性。采用Logistic回归分析筛选术后复发的影响因素。采用受试者工作特征(ROC)曲线研究AIB1 mRNA,ANCO1 mRNA对患者术后复发的预测价值。结果HPV阳性宫颈癌癌组织中AIB1 mRNA(3.04±0.37)高于癌旁组织(0.87±0.21)及HPV阴性宫颈癌癌组织(1.02±0.33),ANCO1 mRNA(1.13±0.26)低于癌旁组织(1.91±0.35)及HPV阴性宫颈癌癌组织(1.82±0.36),差异具有统计学意义(t=68.499,53.137;23.649,17.434,均P<0.05)。HPV阳性宫颈癌癌组织中AIB1 mRNA与ANCO1 mRNA表达呈负相关(r=-0.714,P<0.001)。相比于FIGO分期ⅠA~ⅠB期、无淋巴结转移患者,肿瘤FIGO分期ⅡA期、淋巴结转移HPV阳性宫颈癌患者癌组织中AIB1 mRNA(3.88±0.32 vs 2.04±0.41,4.46±0.33 vs 2.16±0.46)较高,ANCO1 mRNA(0.67±0.29 vs 1.68±0.20,0.49±0.24 vs 1.53±0.32)较低,差异具有统计学意义(t=24.425,26.097;19.288,16.777,均P<0.001)。FIGO分期ⅡA期、淋巴结转移、AIB1 mRNA高水平[OR(95%CI):1.644(1.223~2.210);1.779(1.295~2.444),1.247(1.050~1.728)]是影响HPV阳性宫颈癌患者术后复发的危险因素,ANCO1 mRNA高水平[OR(95%CI):0.634(0.451~0.891)]是保护因素。AIB1mRNA,ANCO1 mRNA联合检测对HPV阳性宫颈癌患者术后复发预测AUC下面积(95%CI)为0.914(0.863~0.952),高于单指标检测[0.821(0.782~0.869),0.794(0.763~0.847)],差异具有统计学意义(Z=4.123,4.432,均P<0.001)。结论HPV阳性宫颈癌中AIB1 mRNA升高,ANCO1Objective To study the expression of amplified in breast cancer 1(AIB1)and ankyrin repeat domain containing11(ANKRD11/ANCO1)in human papillomavirus(HPV)positive cervical cancer and their relationship with clinicopathological characteristics and their predictive value for postoperative recurrence.Methods Cancer tissues and paracancer tissues of 94 HPV positive cervical cancer patients who visited the Third People’s Hospital of Mianyang from January 2018 to January 2020 were selected,while 50 HPV negative cervical cancer tissues during this period were taken as controls.Real-time qPCR(RT-qPCR)was conducted to detect AIB1 mRNA and ANCO1 mRNA expression in cancer tissue and paracancer tissues of HPV positive cervical cancer and cancer tissue of HPV negative cervical.Pearson analysis of the correlation between AIB1 mRNA and ANCO1 mRNA expression in HPV positive cervical cancer tissues.Logistic regression analysis was used to identify the risk factors for postoperative recurrence of HPV-positive cervical cancer patients.The predictive value of AIB1 mRNA,ANCO1 mRNA,and their combination in predicting postoperative recurrence in HPV positive cervical cancer patients was analyzed by receiver operating characteristic curve.Results Compared to the adjacent tissues of HPV positive cervical cancer and cancer tissues of HPV negative cervical cancer,AIB1 mRNA(3.04±0.37 vs 0.87±0.21,1.02±0.33)in HPV positive cervical cancer tissues was higher,while ANCO1 mRNA(1.13±0.26 vs 1.91±0.35,1.82±0.36)was lower,with significant differences(t=68.499,53.137;23.649,17.434,all P<0.05).The expression of AIB1 mRNA and ANCO1 mRNA in HPV positive cervical cancer tissues showed a negative correlation(r=-0.714,P<0.001).Compared to patients with FIGO stageⅡA~ⅡB and without lymph node metastasis,AIB1 mRNA(3.88±0.32 vs 2.04±0.41,4.46±0.33 vs 2.16±0.46)in HPV positive cervical cancer tissues with FIGO stage IIA and lymph node metastasis was higher,while ANCO1 mRNA(0.67±0.29 vs 1.68±0.20,0.49±0.24 vs 1.53±0.32)was lower,with signific

关 键 词：宫颈癌 人乳头状瘤病毒 乳腺癌扩增基因1 锚蛋白重复结构域11 

分 类 号：R737.33[医药卫生—肿瘤] R730.43[医药卫生—临床医学]