蜡样芽孢杆菌TC-1对硫酸盐型盐碱水的生长适应性及其对微囊藻的溶藻效果  

Growth adaptability of Bacillus cereus TC-1 to sulfate-type salinealkaline water and its algicidal effect on Microcystis sp.

作　　者：江昀珈 胡晓娟[2,3] 杨铿 曹煜成[2,3] 许云娜 徐创文[2,4] 张建设 文国樑 JIANG Yunjia;HU Xiaojuan;YANG Keng;CAO Yucheng;XU Yunna;XU Chuangwen;ZHANG Jianshe;WEN Guoliang(National Engineering Research Center for Marine Aquaculture,Zhejiang Ocean University,Zhoushan 316022,China;South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Key Laboratory of South China Sea Fishery Resources Exploitation&Utilization,Ministry of Agriculture and Rural Affairs,Guangdong Provincial Key Laboratory of Fishery Ecology and Environment,Guangzhou 510300,China;Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai),Zhuhai 519000,China;Guangzhou Xinhailisheng Biotechnology Co.,Ltd.Guangzhou 510300,China;East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Shanghai 200090,China)

机构地区：[1]浙江海洋大学国家海洋设施养殖工程技术研究中心,浙江舟山316022 [2]中国水产科学研究院南海水产研究所,农业农村部南海渔业资源开发利用重点实验室,广东省渔业生态环境重点实验室,广东广州510300 [3]南方海洋科学与工程广东省实验室(珠海),广东珠海519000 [4]广州市欣海利生生物科技有限公司,广东广州510300 [5]中国水产科学研究院东海水产研究所,上海200090

出　　处：《中国水产科学》2024年第8期954-965,共12页Journal of Fishery Sciences of China

基　　金：国家重点研发计划项目(2023YFD2401002);中国水产科学研究院基本科研业务费专项资金项目(2023TD57);国家虾蟹产业技术体系项目(CARS-48)。

摘　　要：为研究蜡样芽孢杆菌(Bacillus cereus)菌株TC-1在不同环境条件下的生长适应性及其对两种微囊藻的溶藻效果,开展了不同盐度、pH、温度、碱度条件下菌株的生长适应性研究,并探究其在最适生长条件下,菌株TC-1对铜绿微囊藻(Microcystisaeruginosa)和微囊藻(Microcystissp.)的溶藻效果。结果发现,菌株TC-1在盐度5~35、pH6.0~10.0、温度20~40℃、碱度4.0~16.0mmol/L的条件下生长良好,尤其在盐度15、pH8.0、温度30℃、碱度12.0 mmol/L的条件下生长最好,第8天时细菌密度可达(1.14±0.16)×108 CFU/mL。在碱度4.0 mmol/L和12.0 mmol/L的盐碱水-BG11培养液共培养体系中,溶藻菌菌株TC-1对两种微囊藻的溶藻效果显著,第6天时菌株TC-1对铜绿微囊藻和微囊藻的溶藻率分别可达(82.83±0.03)%和(91.67±0.10)%。结果表明,菌株TC-1对盐度、pH、温度、碱度等环境因子具有良好的适应性,与大部分盐碱池塘水体的盐度、温度、pH的变动区间大体一致;其对微囊藻的溶藻效果良好,可作为盐碱池塘水体防控微囊藻藻华菌剂产品的备选菌株。Saline-alkaline water resources in China are widely distributed.Saline-alkaline water is characterized by a high pH value,carbonate alkalinity,and ion coefficient.Therefore,regulating water quality has become a key challenge and focus of saline-alkaline aquaculture.Frequent outbreaks of harmful Cyanobacteria in saline-alkaline ponds severely affect the healthy growth of aquaculture organisms.Microcystis spp.is one among the major harmful Cyanobacteria.Currently,the primary methods for controlling harmful Cyanobacteria include physical,chemical,and biological methods.However,physical or chemical methods for preventing and controlling algal blooms have certain limitations,such as being time-consuming,labor-intensive,heavy pollutants,and prone to recurrence.Biological methods,such as the use of algicidal bacteria,are highly efficient and safe,making them a hot research topic.In the aquaculture industry,Bacillus species with algicidal properties,such as Bacillus subtilis,Bacillus cereus,Lysinibacillus fusiformis,and Brevibacillus laterosporus are receiving increasing attention from researchers.However,a few reports are available on algicidal bacteria against harmful Cyanobacteria in saline-alkaline ponds.The Ningxia Hui Autonomous Region,located in the northwest of China,has several northwest sulfate-type saline-alkaline ponds.In this study,we selected a strain of B.cereus TC-1,which was isolated and screened from the microalgae environment in saline-alkaline ponds of Ningxia Hui Autonomous Region.The growth adaptability of this strain was studied under different conditions of salinity(5,15,25,and 35),pH(4.0,6.0,8.0,and 10.0),temperature(10℃,20℃,30℃,and 40℃),and alkalinity(4.0 mmol/L,8.0 mmol/L,12.0 mmol/L,and 16.0 mmol/L).Additionally,its algal solubilization effects on two kinds of Microcystis were explored.Using nutrient agar culture medium,the bacterial density of each experimental group was determined on days 0,2,4,6,and 8.The results showed that strain TC-1 grew well under conditions of salinity of 5–

关 键 词：盐碱体系 溶藻菌 微囊藻 蜡样芽孢杆菌 盐碱水 

分 类 号：X52[环境科学与工程—环境工程]