‘巨玫瑰’葡萄叶片原生质体的分离  

Isolation of Protoplasts from Leaves of'Jumeigui'Grapevine

作　　者：刘琰 杨欢 任福仙 谢美玲 房玉林[1] 曾青青 LIU Yan;YANG Huan;REN Fuxian;XIE Meiling;FANG Yulin;ZENG Qingqing(College of Enology,Northwest A&F University,Yangling 712100,China)

机构地区：[1]西北农林科技大学葡萄酒学院,陕西杨凌712100

出　　处：《中外葡萄与葡萄酒》2024年第6期19-24,共6页Sino-Overseas Grapevine & Wine

基　　金：陕西省自然科学基础研究计划项目(2023-JC-QN-0251);西北农林科技大学引进人才科研启动基金项目(Z1090220307)。

摘　　要：以‘巨玫瑰’葡萄茎段为试材进行组培苗培养,通过调节IBA浓度,探究其生根诱导的最适培养基。并在最适培养基条件下,以无菌苗叶片为材料,对原生质体分离条件进行研究,为细胞融合、基因工程及品种改良等研究奠定基础。结果表明,IBA浓度对茎段生根有显著影响,以0.4 mg·L^(-1)时生根效果最好,根长为2.91 cm,增长系数为0.86;酶种类和浓度、酶解时间对原生质体分离有显著影响,采用40 g·L^(-1)纤维素酶+15 g·L^(-1)离析酶+5 g·L^(-1)果胶酶的酶液组合、酶解时间为2 h时,原生质体的产量和活性均达到最佳,分别为每克3.20×10^(6)个和82.88%。The stem segment of'Jumeigui'grapevine was used for tissue culture,and the optimal medium for rooting induction was explored by adjusting the concentration of IBA.Under the optimum medium condition,the protoplast separation conditions were studied by using the leaves of sterile seedlings as the material,which laid the foundation for research of cell fusion,genetic engineering and variety improvement.The results showed that the concentration of IBA had a significant effect on stem rooting,the optimal rooting concentration was 0.4 mg·L^(-1),the rooting length was 2.91 cm,and the growth coefficient was 0.86.The types,concentration and time of enzymatic hydrolysis had obvious effect on the protoplast separation.When the enzymatic hydrolysate solution was combined with 40 g·L^(-1)cellulase,15 g·L^(-1)macerozyme and 5 g·L^(-1)pectinase for 2 h,the yield and activity of protoplasts was the best,which were 3.20×10^(6)cells·g^(-1)and 82.88%,respectively.

关 键 词：巨玫瑰 原生质体 葡萄叶片 分离 

分 类 号：S663.1[农业科学—果树学]