激光共聚焦显微镜样本制备在研究生实验教学中的应用  

The Application of Laser Scanning Confocal Microscope Sample Preparation in Postgraduate Experimental Teaching

作　　者：赵新英 李佩茹 Zhao Xinying;Li Peiru(College of Life Science,Shandong Agricultural University,Tai'an,271018,China;Tai'an Academy of Agricultural Sciences,Tai'an,271000,China)

机构地区：[1]山东农业大学生命科学院,山东泰安271018 [2]泰安市农业科学院,山东泰安271000

出　　处：《中国现代教育装备》2024年第21期24-26,共3页China Modern Educational Equipment

基　　金：国家自然科学青年基金“拟南芥RAB5调控花粉管生长的功能和机理研究”(编号:32100296);山东省优质研究生课程建设项目(编号:SDYKC2022033)。

摘　　要：激光共聚焦显微镜是一种多功能、高分辨率、高效率的光学设备,在植物细胞结构中通过荧光探针标记相关基因,获得清晰的亚细胞定位信息,如细胞膜、细胞器等结构。以烟草为实验材料,在研究生实验教学中利用LSCM880激光扫描共聚焦显微镜观察烟草下表皮细胞,通过优化样本制作,最终获取清晰的图片。通过实验教学,学生掌握激光共聚焦显微镜的原理、样品制备及操作步骤,提高理论知识与实践操作相结合的能力,加强其对激光共聚焦显微镜的掌握及运用。Laser scanning confocal microscopy is a multi-functional,high-resolution and efficient optical instrument.By labeling target genes with fluorescent probes in plants,it can obtain clear positioning information of subcellular structures such as cell membrane or organelles.In this article,tobacco is used as experimental material,and LSCM880 laser scanning confocal microscopy is used to observe tobacco lower epidermal cells in postgraduate experimental teaching.Throughthe experimental teaching,the postgraduates enable to master the principle of confocal microscopy,sample preparation and utilization of LSCM.This is intended to cultivate the students'ability to apply theoretical knowledge to practical situations,while at the same time enabling them to understand the appilcation of the LSCM in plant science.

关 键 词：激光共聚焦显微镜 实验教学 样本制备 烟草 

分 类 号：Q-33[生物学] G642[文化科学—高等教育学]