初诊急性髓系白血病患者体内MAIT细胞的表达和功能  

Expression and Function of MAIT Cells in Patients with Newly Diagnosed Acute Myeloid Leukemia

作　　者：彭谦 王芝涛 黄仁华 王会平[1] 肖浩 翟志敏[1] PENG Qian;WANG Zhi-Tao;HUANG Ren-Hua;WANG Hui-Ping;XIAO Hao;ZHAI Zhi-Min(Department of Hematology,The Second Hospital of Anhui Medical University,Hefei Anhui Province,China)

机构地区：[1]安徽医科大学第二附属医院血液内科,安徽合肥230601

出　　处：《中国实验血液学杂志》2024年第6期1644-1650,共7页Journal of Experimental Hematology

基　　金：国家自然科学基金资助项目(82200225)。

摘　　要：目的:探讨初诊急性髓系白血病(AML)患者外周血黏膜相关恒定T(MAIT)细胞数量与免疫功能的变化,及其与AML发生、发展的关系。方法:选择2022年1月-2023年2月本院75例初诊AML患者临床样本以及48例健康对照样本作为研究对象,采用多参数流式细胞术检测样本中的MAIT细胞数量、膜表面标志物、效应表型以及功能指标。结果:与健康对照相比,初诊AML患者外周血MAIT细胞占CD3^(+)T细胞比例显著降低(P<0.001)。患者骨髓中的MAIT细胞占全部CD3+T细胞比例与外周血类似(P>0.05)。AML患者外周血MAIT细胞多为效应记忆型T细胞,且与健康对照相比,效应记忆型MAIT细胞比例降低(P<0.05),而终末分化效应记忆型MAIT细胞与PD-1+MAIT细胞比例明显增高(均P<0.05)。AML患者外周血MAIT细胞表达颗粒酶B、穿孔素的水平明显高于健康对照(均P<0.05),分泌γ干扰素、肿瘤坏死因子α等细胞因子的水平明显低于健康对照(均P<0.001)。结论:AML患者体内MAIT细胞比例降低且功能标记物表达异常,提示其功能受损,可能参与AML的发生、发展。Objective:To explore the changes in number and immune function of mucosal-associated invariant T(MAIT)cells in peripheral blood of patients with newly diagnosed acute myeloid leukemia(AML),and its correlation with the occurrence and development of AML.Methods:Seventy-five clinical samples of patients with newly diagnosed AML and 48 healthy control samples in our hospital from January 2022 to February 2023 were included.Multiparametric flow cytometry was used to detect the number of MAIT cells,membrane surface markers,effector phenotypes and functional indicators in the samples.Results:Compared with healthy controls,the percentage of MAIT cells in+CD3 T cells in peripheral blood of newly diagnosed AML patients was significantly reduced(P<0.001).The percentage of MAIT cells in all CD3^(+)T cells in bone marrow of AML patients was similar to that in peripheral blood(P>0.05).Most of MAIT cells in peripheral blood of AML patients were effector memory T cells.Compared with healthy controls,the proportion of effector memory MAIT cells decreased(P<0.05),while the proportion of terminally differentiated effector memory MAIT cells and PD-1+MAIT cells increased significantly(both P<0.05).AML patients'peripheral blood MAIT cells expressed significantly higher levels of granzyme B and perforin than healthy controls(both P<0.05),and secreted significantly lower levels of cytokines such as gamma interferon and tumor necrosis factorαthan healthy controls(both P<0.001).Conclusion:Compared with healthy controls,the proportion of MAIT cells in AML patients is reduced and the expression of functional markers is abnormal,suggesting that their function is impaired and may be involved in the occurrence and development of AML.

关 键 词：急性髓系白血病 免疫细胞 黏膜相关恒定T细胞 程序性死亡受体1 细胞因子 

分 类 号：R733.71[医药卫生—肿瘤]