多发性骨髓瘤细胞表达miR-383-5p的研究及临床意义  

Expression and Clinical Significance of miR-383-5p in Multiple Myeloma Cells

作　　者：刘林 杨艳丽 张凤 李佳佳 LIU Lin;YANG Yan-Li;ZHANG Feng;LI Jia-Jia(Department of Hematology,The First Affiliated Hospital of Bengbu Medical University,Bengbu 233000,Anhui Province,China)

机构地区：[1]蚌埠医科大学第一附属医院血液科,安徽蚌埠233000

出　　处：《中国实验血液学杂志》2024年第6期1771-1775,共5页Journal of Experimental Hematology

基　　金：蚌埠医学院自然科学重点项目(2022byzd048)。

摘　　要：目的:探讨miR-383-5p在初治多发性骨髓瘤(MM)中的表达及其与临床特征及预后的相关性。方法:回顾性收集2013年1月至2017年1月蚌埠医科大学第一附属医院血液科确诊及治疗的初治MM患者及非肿瘤对照者各115例,收集患者临床特征、病理资料及治疗反应,通过RT-qPCR检测miR-383-5p在MM患者及非肿瘤对照者中的表达情况,进一步分析miR-383-5p表达量与MM患者临床特征及预后的关系。结果:RT-qPCR结果显示,115例非肿瘤对照者骨髓组织与115例MM患者骨髓组织中miR-383-5p相对表达量分别为1.89±0.11与1.48±0.13,比较差异有统计学意义(P<0.05)。卡方检验结果提示,miR-383-5p表达与骨损伤、β2-微球蛋白和球蛋白相关(均P<0.001),但与其他因素包括年龄、性别、血红蛋白和轻链不相关。单变量与多变量Cox回归分析表明,低表达miR-383-5p是MM患者OS不良的独立危险因素(P<0.001)。结论:miR-383-5p在MM患者中表达下调,且低表达miR-383-5p是预后不良的危险因素,可作为新的预后生物标志物。Objective:To investigate the expression of miR-383-5p in newly diagnosed multiple myeloma(MM)and its correlation with clinical features and prognosis.Methods:115 MM patients diagnosed and treated in the Department of Hematology,the First Affiliated Hospital of Bengbu Medical University from January 2013 to January 2017 and 115non-tumor controls were enrolled in this study.Clinical characteristics,pathological data and therapeutic responses of the patients were collected.The expression of miR-383-5p in MM patients and non-tumor controls was detected by RT-qPCR,and the relationship between the expression level of miR-383-5p and the clinical characteristics and prognosis of MM patients was further analyzed.Results:The results of RT-qPCR showed that the relative expression levels of miR-383-5p in bone marrow tissues of 115 non-tumor controls and 115 MM patients were 1.89±0.11 and 1.48±0.13,respectively,and the difference was statistically significant(P<0.05).Chi-square analysis showed that the expression of miR-383-5p was correlated with bone injury,β2-microglobulin and globulin(all P<0.001),but not with other factors including age,sex,hemoglobin and light chain.Univariate and multivariate Cox regression analysis indicated that low expression of miR-383-5p was an independent risk factor for poor OS in MM patients(P<0.001).Conclusion:The expression of miR-383-5p is down-regulated in MM patients,and low expression of miR-383-5p is a risk factor for poor prognosis and can be used as a new prognostic biomarker.

关 键 词：多发性骨髓瘤 miR-383-5p 预后 

分 类 号：R733.3[医药卫生—肿瘤]