NCAPG和CDK1促进膀胱癌细胞增殖  

NCAPG and CDK1 promote bladder cancer cell proliferation

作　　者：李长建[1] 王会青 王玉华[1] 王亚林 窦启锋[2] LI Changjian;WANG Huiqing;WANG Yuhua;WANG Yalin;DOU Qifeng(Department of Urology,The Third Affiliated Hospital of Xinxiang Medical College,Xinxiang 453000,China;Department of Urology,The First Affiliated Hospital of Xinxiang Medical College,Weihui 453100,China)

机构地区：[1]新乡医学院第三附属医院泌尿外科,新乡453000 [2]新乡医学院第一附属医院泌尿外科,卫辉453100

出　　处：《天津医科大学学报》2024年第6期491-496,502,共7页Journal of Tianjin Medical University

摘　　要：目的:探讨非SMC凝缩蛋白Ⅰ复合物亚基G(NCAPG)和细胞周期蛋白依赖性激酶1(CDK1)在膀胱癌细胞和膀胱癌组织中的作用。方法:通过生物信息学分析,比较NCAPG和CDK1在膀胱癌和正常组织中的表达。之后再对NCAPG和CDK1进行相关性分析,对新乡医学院第一和第三附属医院泌尿外科72例膀胱癌患者术后病理组织进行免疫组化染色,进一步验证其相关性。选取膀胱癌细胞T24和5637,将其分为两组,未敲低NCAPG的为对照组,转染shRNA敲低NCAPG的为shRNA组,qRT-PCR和Western印迹验证敲低效果,通过克隆形成和CCK-8实验检测其对膀胱癌细胞增殖的影响,并应用细胞周期实验,检测其对膀胱癌细胞周期的影响。结果:与正常膀胱组织相比,NCAPG和CDK1在膀胱癌组织中表达上调。NCAPG和CDK1免疫组化染色的相关性分析进一步证实了NCAPG与CDK1相关(χ^(2)=10.286,P<0.001)。通过结合临床资料进一步发现,NCAPG的表达与肿瘤大小密切相关(χ^(2)=6.675,P=0.010)。qRT-PCR和Western印迹结果显示,NCAPG shRNA明显抑制了NCAPG mRNA(t=5.422、5.238,均P<0.05)及蛋白的表达(t=4.756、4.122,均P<0.05)。同时CCK-8实验和克隆形成实验发现,敲低NCAPG会抑制膀胱癌细胞的增殖(t=5.886、4.147、3.102、3.745,均P<0.05)。细胞周期结果发现,敲低NCAPG后阻滞了膀胱癌细胞的G2/M期(t=2.566、2.926,均P<0.05)。结论:NCAPG和CDK1在膀胱癌细胞中高表达,会促进膀胱癌细胞的增殖。Objective:To investigate the role of the non-SMC condensinⅠcomplex subunit G(NCAPG)and cyclin dependent kinase 1(CDK1)in bladder cancer cells and bladder cancer tissues.Methods:Bioinformatics analysis was used to compare the expression of NCAPG and CDK1 in bladder cancer and normal tissues.The correlation between NCAPG and CDK1 was analyzed,and immunohistochemical staining was performed on the pathological tissues of 72 patients with bladder cancer after surgery in our hospital to further verify their correlation.Bladder cancer cells T24 and 5637 were selected and divided into two groups.The control group was the one that did not knock down NCAPG,and the shRNA group was the one that transfected shRNA to knock down NCAPG.QRT-PCR and Western blotting were used to verify the knockdown effect,clone formation and CCK8 assay were used to detect the effect of NCAPG on bladder cancer cell proliferation,and the cell cycle experiment was used to detect the effect on the cell cycle of bladder cancer.Results:NCAPG and CDK1 were up-regulated in bladder cancer tissues compared with normal bladder tissues.And the correlation analysis of NCAPG and CDK1 immunohistochemical staining further confirmed that NCAPG was related to CDK1(χ^(2)=10.286,P<0.001).Combined with clinical data,it was further found that the expression of NCAPG was closely related to tumor size(χ^(2)=6.675,P=0.010).QRT-PCR and Western blotting results showed that NCAPG shRNA significantly inhibited the expression of NCAPG mRNA(t=5.422,5.238,both P<0.05)and protein(t=4.756,4.122,both P<0.05).Meanwhile,knockdown of NCAPG inhibited the proliferation of bladder cancer cells according to colony formation and CCK8 assay(t=5.886,4.147,3.102,3.745,all P<0.05).Cell cycle results showed that NCAPG knockdown blocked the G2/M phase of bladder cancer cells(t=2.566,2.926,both P<0.05).Conclusion:NCAPG and CDK1 are highly expressed in bladder cancer cells and can promote the proliferation of bladder cancer cells.

关 键 词：NCAPG CDK1 膀胱癌 治疗靶点 增殖 

分 类 号：R737.14[医药卫生—肿瘤]