肺腺癌组织中乙醛脱氢酶2降低的机制及其与患者预后的关系  

Mechanism of Decreased Aldehyde Dehydrogenase 2 in Lung Adenocarcinoma Tissue and Its Relationship with Patient Prognosis

作　　者：孟令云 范婧 王育梅 徐非非 胥燕 赵明宏 袁琳 MENG Lingyun;FAN Jing;WANG Yumei;XU Feifei;XU Yan;ZHAO Minghong;YUAN Lin(Department of Medical Oncology,Jianhu Clinical Medical College,Yangzhou University/Affiliated Hospital of Nantong University Xinglin College,Yancheng 224700,China)

机构地区：[1]扬州大学建湖临床医学院、南通大学杏林学院附属医院肿瘤内科,江苏省盐城市224700

出　　处：《实用心脑肺血管病杂志》2024年第12期80-87,共8页Practical Journal of Cardiac Cerebral Pneumal and Vascular Disease

基　　金：江苏省卫生健康委医学科研重点项目(ZD2021034);2023年度省科技计划专项资金拟立项目(BK20231207)。

摘　　要：目的探讨肺腺癌组织中乙醛脱氢酶2(ALDH2)降低的机制及其与患者预后的关系。方法(1)数据库分析。采用UALCAN数据库分析肺腺癌组织及癌旁组织中ALDH2 mRNA表达每百万转录本和蛋白表达Z分数;采用甲基化数据可视化工具(SMART)分析泛癌模块、4种探针肺腺癌组织中和癌旁组织中ALDH2 DNA甲基化水平;采用基因表达谱互动分析(GEPIA)数据库分析肺腺癌组织中ALDH2 mRNA表达水平与细胞周期调控蛋白表达水平的相关性;采用TIMER 2.0数据库中数据分析肺腺癌组织中ALDH2 mRNA表达水平与6种免疫细胞浸润丰度的相关性。(2)临床试验。回顾性选取2019年1月—2023年1月南通大学杏林学院附属医院收治的行手术治疗的肺腺癌患者55例为研究对象,收集患者的年龄、性别、吸烟史、肿瘤大小、肿瘤分期、手术类型、病理类型,采用免疫组织化学法检测肺腺癌组织及癌旁组织中ALDH2表达水平。对所有患者进行随访,随访截止时间为2024年1月,主要结局事件为死亡。采用Spearman秩相关分析探讨肺腺癌组织中ALDH2 mRNA表达水平与细胞周期调控蛋白表达水平、免疫细胞浸润丰度的相关性;采用单因素、多因素Cox比例风险回归分析探讨肺腺癌患者预后的影响因素;采用K-M法绘制生存曲线,生存率比较采用Log-rank检验。结果(1)数据库分析结果。UALCAN数据库分析结果显示,肺腺癌组织中ALDH2 mRNA表达每百万转录本和蛋白表达Z分数低于癌旁组织(P<0.05)。SMART分析结果显示,泛癌模块肺腺癌组织中ALDH2 DNA甲基化水平较癌旁组织升高(P<0.05);4种探针(cg07106761、cg20884605、cg05212570、cg19848940)肺腺癌组织中ALDH2 DNA甲基化水平较癌旁组织升高(P<0.05)。Spearman秩相关分析结果显示,肺腺癌组织中ALDH2 mRNA表达水平与CDCA2、DEPDC1、ESPL1、EXO1、FAM83D、FANCI、FOXM1、HJURP表达水平呈负相关(P<0.05);肺腺癌组织中ALDH2 mRNA表达水平与B淋巴�Objective To investigate the mechanism of decreased aldehyde dehydrogenase 2(ALDH2)in lung adenocarcinoma tissue and its relationship with patient prognosis.Methods(1)Database analysis.UALCAN database was used to analyze the ALDH2 mRNA expression per million transcripts and protein expression Z-score in lung adenocarcinoma tissues and paracancerous tissues;the Shiny Methylation Analysis Resource Tool(SMART)was used to analyze the ALDH2 DNA methylation levels in pan-cancer module,four probes of lung adenocarcinoma tissues,and paracancerous tissues;the Gene Expression Profiling Interactive Analysis(GEPIA)database was used to analyse the correlation between the ALDH2 mRNA expression level and cell-cycle-regulated proteins expression level in lung adenocarcinoma tissues;and the data in TIMER 2.0 database were used to analyze the correlation between ALDH2 mRNA expression level and the abundance of six immune cell infiltrations in lung adenocarcinoma tissues.(2)Clinical trial.A total of 55 patients with lung adenocarcinoma undergoing surgical treatment admitted to Affiliated Hospital of Nantong University Xinglin College from January 2019 to January 2023 were retrospectively selected as the study objects,and the age,gender,smoking history,tumour size,tumour stage,surgery type,and pathology type of patients were collected,and immunohistochemistry was used to detect ALDH2 expression level in the tissues of lung adenocarcinoma and the paracancerous tissues.All patients were followed up,and the follow-up cut-off was January 2024,and the primary outcome event was death.Spearman rank correlation analysis was used to explore the correlation between the ALDH2 mRNA expression level and the abundance of cell-cycle-regulated proteins expression level and immune cell infiltration in lung adenocarcinoma tissues.Univariate and multivariate Cox proportional risk regression analysis were used to explore the influncing factors of the prognosis in patients with lung adenocarcinoma;the K-M method was used to draw survival curves,and surv

关 键 词：肺腺癌 乙醛脱氢酶2 预后 

分 类 号：R734.2[医药卫生—肿瘤]