CENPM基因表达下调的前列腺癌PC3细胞增殖、凋亡、侵袭能力及EMT表型变化  

Effect of down-regulated CENPM gene expression on proliferation,apoptosis,invasion,and EMT phenotype changes of prostate cancer PC3 cells

作　　者：张磊 何泽来 ZHANG Lei;HE Zelai(Department of Radiotherapy,The First Affiliated Hospital of Bengbu Medical University,Bengbu 233004,China)

机构地区：[1]蚌埠医科大学第一附属医院放疗科,安徽蚌埠233004

出　　处：《山东医药》2024年第32期38-41,共4页Shandong Medical Journal

基　　金：蚌埠医学院自然科学研究重点项目(2023byzd091)。

摘　　要：目的观察着丝粒蛋白M(CENPM)基因表达下调对前列腺癌PC3细胞增殖、凋亡、侵袭能力及上皮—间充质转化(EMT)表型的影响。方法体外培养人前列腺癌PC3细胞并分为实验组和对照组,实验组转染CENPM shRNA,对照组转染阴性对照shRNA。采用CCK-8法检测细胞增殖能力并计算增殖率,采用流式细胞术检测细胞凋亡情况并计算凋亡率,采用Transwell实验检测细胞侵袭能力,采用Western blotting法检测EMT相关蛋白E-cadherin、Vimentin、N-cadherin。结果实验组细胞增殖率、侵袭能力及Vimentin、N-cadherin蛋白表达低于对照组,细胞凋亡率及E-cadherin蛋白表达高于对照组(P均<0.05)。结论CENPM基因表达下调可降低前列腺癌PC3细胞的增殖和侵袭能力,抑制EMT,促进细胞凋亡。Objective To observe the effects of down-regulated centromere protein M(CENPM)gene expression on the proliferation,apoptosis,invasion ability,and epithelial-mesenchymal transition(EMT)phenotype of prostate cancer PC3 cells.Methods Human prostate cancer PC3 cells were cultured in vitro and divided into the experimental group and control group.The experimental group was transfected with CENPM shRNA,while the control group was transfected with negative control shRNA.The cell proliferation ability was measured using the CCK-8 assay,and the proliferation rate was calculated.Apoptosis was detected using flow cytometry,and the apoptosis rate was calculated.The invasion ability of the cells was assessed using Transwell assay.EMT-related proteins(E-cadherin,Vimentin,and N-cadherin)were detected by Western blotting.Results The experimental group showed lower cell proliferation rate,invasion ability,and expression levels of Vimentin and N-cadherin proteins as compared with the control group;in contrast,the apoptosis rate and E-cadherin protein expression were higher in the experimental group than in the control group(all P<0.05).Conclusion Downregulation of CENPM gene expression can reduce the proliferation and invasion of prostate cancer PC3 cells,inhibit EMT,and promote apoptosis.

关 键 词：CENPM基因 前列腺肿瘤 细胞增殖 细胞凋亡 肿瘤浸润 上皮—间充质转化 

分 类 号：R737.25[医药卫生—肿瘤]