奥氮平干预的脂肪细胞炎症因子分泌水平观察  

Secretion levels of inflammatory cytokines in adipocytes intervened by olanzapine

作　　者：杜晓尧 钱艳伟 姚庆兄 徐广峰 DU Xiaoyao;QIAN Yanwei;YAO Qingxiong;XU Guangfeng(The Second Clinical Medical College,Xuzhou Medical University,The Third People′s Hospital of Huai′an City,Huai′an 223001,China)

机构地区：[1]徐州医科大学淮安第二临床学院江苏省淮安市第三人民医院检验科,江苏淮安223001

出　　处：《山东医药》2024年第32期47-50,共4页Shandong Medical Journal

基　　金：国家自然科学基金青年基金项目(81600689);江苏省淮安市科技计划项目(HAB202124)。

摘　　要：目的观察奥氮平对脂肪细胞分泌炎症细胞因子水平的影响。方法采用1-甲基-3-异丁基黄嘌呤、地塞米松、胰岛素诱导3T3-L1前体脂肪细胞向成熟脂肪细胞分化,将成熟脂肪细胞分为实验1组、实验2组、实验3组和对照组。实验1组、实验2组、实验3组培养基中分别加入2.5、5.0、10.0μmol/L的奥氮平干预细胞,对照组不加奥氮平,正常培养。分别收集干预12、24、48、72 h后的细胞培养液上清,采用ELISA法检测白细胞介素(IL)-6、IL-10和肿瘤坏死因子α(TNF-α)。结果各组细胞培养液上清IL-6、IL-10水平随干预时间延长而增高(P均<0.05)。各实验组干预72 h细胞培养液上清IL-6水平均高于对照组,实验2组高于实验1组和实验3组(P均<0.05)。实验1组、实验2组干预48、72 h细胞培养液上清IL-10水平低于对照组,实验3组干预72 h的IL-10水平低于对照组(P均<0.05);实验1组、实验2组72 h培养液上清IL-10水平低于实验3组(P均<0.05)。各组干预不同时点细胞培养液上清TNF-α吸光度值均较低,无法根据标准曲线法计算获取浓度。结论奥氮平可促进脂肪细胞分泌促炎因子IL-6,抑制抗炎因子IL-10分泌,这些作用可能与奥氮平治疗的精神分裂症患者发生肥胖有关。Objective To explore the effect of different concentrations of olanzapine on secretion of inflammatory cytokines in adipocytes.Methods 3T3-L1 cells were induced to mature adipocytes using 3-Isobutyl-1-methylxanthine,dexamethasone,and insulin.And then,mature adipocytes were randomly divided into the experiment group 1,experiment group 2,experiment group 3 and control group,respectively.Cells in the experiment groups were intervened with 2.5,5.0,and 10μmol/L olanzapine,respectively.Cells in the control group were cultured normally without olanzapine.We collected the supernatants of the cell culture medium after intervention for 12,24,48 and 72 h,respectively.The levels of inflammatory cytokines,interleukin-6(IL-6),interleukin-10(IL-10)and tumor necrosis factor-α(TNF-α)were measured by ELISA.Results IL-6 and IL-10 in culture supernatant of mature adipocytes in all experiment groups increased over the intervention time(all P<0.05).The expression of IL-6 was higher in all experiment groups at 72 h after olanzapine treatment in comparison with that of the control group;that was higher in the experiment group 2 than in the experiment group 1 and experiment group 3 at 72 h after olanzapine treatment(all P<0.05).The expression levels of IL-10 were lower in the experiment group 1 and experiment group 2 than in the control group after olanzapine intervention for 48 or 72 h,and the expression of IL-10 was lower in the experiment group 3 than in the control group after olanzapine intervention for 72 h(all P<0.05);the expression of IL-10 was lower in the experiment group 1 and experiment group 2 than in the experiment group 3 after olanzapine intervention for 72 h(both P<0.05).However,the absorbance values of TNF-αin the supernatants of the cell culture medium at different time points of intervention in each group were all relatively low,and it was not possible to calculate and obtain the concentrations according to the standard curve method.Conclusions Olanzapine can promote the secretion of pro-inflammatory cytokines IL-

关 键 词：奥氮平 3T3-L1细胞 炎症因子 肥胖 精神分裂症 

分 类 号：R394[医药卫生—医学遗传学]