Multifunctional Ce-MOF@PdNPs with colorimetric fluorescent electrochemical activity for ultrasensitive and accurate detection of diethylstilbestrol  

作　　者：Mingyue Ye Tingting Su Jin Li Xiaowan Chen Dichen Ying Shijia Wu Zhouping Wang Nuo Duan 

机构地区：[1]State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, 214122, China [2]School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China [3]International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, 214122, China

出　　处：《Nano Research》2024年第11期9990-9998,共9页纳米研究（英文版）

基　　金：supported by Jiangsu Agriculture Science and Technology Innovation Fund(No.CX(22)3006);National Natural Science Foundation of China(Nos.32272449 and 32072310);the Fundamental Research Funds for the Central Universities(No.JUSRP622025);Collaborative innovation center of food safety and quality control in Jiangsu Province.

摘　　要：The development of biosensors is gaining tremendous attention in various fields due to their extraordinary advantages, however, their sensitivity and accuracy are still challenging. Herein, we proposed a novel multifunctional nanocomposite Ce-MOF@PdNPs (MOF = metal-organic framework, PdNPs = Pd nanoparticles)-mediated triple-readout aptasensor for accurate and reliable detection of diethylstilbestrol (DES), in which Ce-MOF@PdNPs exhibited excellent peroxidase (POD)-like activity, fluormetric, and electro conductive properties. In addition, enzymes-assisted target recycling amplification was utilized to improve the sensitivity, that is the specific binding of aptamer and DES triggered an Exo III enzyme-assisted recycling reaction. The generated F-DNA was captured by the H3 strand linked to Ce-MOF@PdNPs immobilized on the electrode, exposing cleavage sites and activating the Nt.BbvCI enzyme-assisted recycling reaction, leading to the dissociation of Ce-MOF@PdNPs and a significant reduced electrochemical signal. The collected Ce-MOF@PdNPs solution also induced a proportional change in the color and fluorescence, achieving a colorimetric and fluormetric detection functionality. The detection limit under colorimetric mode was 0.16 and 0.76 ng/mL under fluorescence mode, and 0.87 pg/mL under electrochemical mode. This triple-readout aptasensor exhibits high sensitivity, selectivity and accuracy, providing a new idea for designing novel biosensing platforms for veterinary drug residue detection.

关 键 词：cerium-based materials Pd nanoparticles(PdNPs) APTAMER colorimetric/fluorescence/electrochemical signal amplification 

分 类 号：O64[理学—物理化学]