云南省熟肉制品中沙门氏菌、致泻大肠埃希氏菌污染状况调查  

Survey on the contamination of Salmonella and diarrheagenicEscherichia coli in cooked meat products in Yunnan Province

作　　者：杨菁 罗荣爱 马妮 魏振燕 吴林蔚 汤晓召 YANG Jing;LUO Rong’ai;MA Ni;WEI Zhenyan;WU Linwei;TANG Xiaozhao(Yunnan Center for Disease Control and Prevention,Kunming,Yunnan 650022,China;不详)

机构地区：[1]云南省疾病预防控制中心,云南昆明650022 [2]玉溪市疾病预防控制中心

出　　处：《中国国境卫生检疫杂志》2024年第5期463-467,480,共6页Chinese Journal of Frontier Health and Quarantine

摘　　要：目的了解、分析云南省熟肉制品中沙氏门菌、致泻大肠埃希氏菌的污染状况与耐药情况,并分析耐药基因、毒力基因。方法2022年采集云南省范围内不同类别、不同经营环节的熟肉制品296件,依据食品安全国家标准检测样品中沙门氏菌、致泻大肠埃希氏菌,并根据CLSI中的微量肉汤稀释法对阳性菌进行药敏检验,与耐药基因库Res-Finder4.1、毒力基因库VFDB进行比对。结果2022年在云南省采集的296件熟肉制品中沙门氏菌和致泻大肠埃希氏菌的检出率分别为3.72%(11/296)、4.73%(14/296)。沙门氏菌对四环素、氨苄西林、米诺环素耐药率高,致泻大肠埃希氏菌对萘啶酸、四环素和头孢唑林耐药率高。所有沙门氏菌均携带aac(6')-Iaa耐药基因,3株致泻大肠埃希氏菌未携带相关的耐药基因,1株沙门氏菌携带spvB、cdtB、pltA、pltB毒力基因,1株致泻大肠埃希氏菌携带spvB、cdtB、pltA、pltB毒力基因。结论云南省熟肉制品致病菌污染情况较严重,沙门氏菌和致泻大肠埃希氏菌耐药严重,应加强监管。Objective To understand and analyze the contamination status and drug resistance of Salmonella and diarrheagenic Escherichia coli(E.coli)in cooked meat products in Yunnan Province,and to analyze the drug resistance genes and virulence genes.Methods In 2022,296 pieces of cooked meat products in different categories and different operating segments in Yunnan were collected,Salmonella and diarrheagenic E.coli were detected according to the national food safety standards.The drug sensitivity test was performed on the isolated bacteria according to the micro-broth dilution method in CLSI.The gene sequences obtained were analyzed with drug resistance gene database ResFinder4.1 and virulence gene database VFDB.Results The positive rates of Salmonella and diarrheagenic E.coli were 3.72%(11/296),4.73%(14/296)among 296 cooked meat products collected in Yunnan during 2022.Salmonella was highly resistant to tetracycline,ampicillin and minocycline,diarrheagenic E.coli was highly resistant to nalidixic acid,tetracycline and cefazolin.All Salmonella strains carried aac(6')-Iaa resistant gene,and 3 strains of diarrheagenic E.coli did not carry any of the resistant gene.One strain of Salmonella carried virulence genes which inclued spvB,cdtB,pltA and pltB.One strain of diarrheagenic E.coli carried virulence genes which inclued spvB,cdtB,pltA,and pltB.Conclusion There was a greater contamination of pathogenic bacteria in cooked meat products in Yunnan.The drug resistance of Salmonella and diarrheagenic E.coli were serious.It was should strengthen the supervision.

关 键 词：沙门氏菌 致泻大肠埃希氏菌 耐药 基因分析 

分 类 号：R183.4[医药卫生—流行病学] R155[医药卫生—公共卫生与预防医学]