The anti-tumor and mechanism of the benzoisoselenazolone derivatives on the human lung cancer adenocarcinoma A549 cells  

作　　者：Xiang-Bo Gou Yun-Sheng Xu Chen-Yue Shao Xin-Ru Zhao Zhuo Zhang Yan-Ping Shi Ying-Jie Lei 

机构地区：[1]School of Chemistry and Chemical Engineering,Tianjin University of Technology,Tianjin 300384,China.

出　　处：《Biomedical Engineering Communications》2024年第4期20-28,共9页生物医学工程通讯

基　　金：supported by the National Natural Science Foundation of China(NSFC)Youth Project(No.82204397).

摘　　要：Background:In this research,we investigated the anti-cancer effect and the related mechanism of 2-[2-(4-chlorobenzamidomethylthio)-1,3,4-thiadiazol-5-yl]-1,2-benziselenazol-3(2H)-one compound(CTBO)and 2-[2-(4-nitrobenzamidomethylthio)-1,3,4-thiadiazol-5-yl]-1,2-benziselenazol-3(2H)-one compound(NTBO),which we synthesized in our lab previously.Methods:We applied the human lung cancer adenocarcinoma A549 cells to investigate the anti-tumor effect of CTBO and NTBO.The following methods were used in the research,including methylthiazolyldiphenyl-tetrazolium bromide assay,one-step terminal-deoxynucleotidyl transferase mediated nick end labeling,transcriptome sequencing analysis,quantitative reverse transcription polymerase chain reaction and western blot.Results:The results showed that both CTBO and NTBO significantly inhibited the A549 cells proliferation and induced the A549 cells apoptosis.The transcriptome sequencing analysis results illustrated that the two derivatives might exert the apoptotic effects through mitogen-activated protein kinase and tumor necrosis factor signaling pathways activation.Further,the western blot results suggested that CTBO and NTBO exerted anti-cancer effect through different molecular mechanisms.Conclusion:The results above provided fundamental research evidence for the further application of benziselenazolone derivatives in clinical.

关 键 词：benziselenazolone derivatives lung cancer mitogen-activated protein kinase signaling pathways tumor necrosis factor signaling pathways APOPTOSIS 

分 类 号：R73[医药卫生—肿瘤]