溴氰虫酰胺聚乳酸微胶囊的制备及性能评价  

Preparation and performance evaluation of cyantraniliprole polylactic acid microcapsules

作　　者：刘贵莹 周芷若 陈惠玲 张蕊 张贺[2] 杨石有 LIU Guiying;ZHOU Zhiruo;CHEN Huiling;ZHANG Rui;ZHANG He;YANG Shiyou(College of Biological and Agricultural Sciences,Honghe University,Mengzi 661199,Yunnan,China;Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China)

机构地区：[1]红河学院生物科学与农学学院,云南蒙自661199 [2]中国热带农业科学院环境与植物保护研究所,海口571101

出　　处：《农药》2024年第11期812-819,842,共9页Agrochemicals

基　　金：云南省教育厅科学研究基金项目(2023J1117);云南省大学生创新创业训练计划项目(CX2022209);红河学院科研基金博士专项(XJ22B16)。

摘　　要：[目的]针对溴氰虫酰胺农药利用率低和对非靶标生物急性毒性高等问题,制备性能较好的溴氰虫酰胺聚乳酸微胶囊,以期为该药剂的使用提供参考。[方法]以溴氰虫酰胺为芯材,聚乳酸为壁材,采用溶剂挥发法制备溴氰虫酰胺聚乳酸微胶囊。通过单因素试验考查不同芯壁材质量比例、壁材浓度、油水相比例、乳化剪切速度、磁力搅拌速度及搅拌时间对溴氰虫酰胺微胶囊载药量、包封率、粒径的影响,同时以载药量、包封率为评价指标,采用L16(45)正交试验对微胶囊制备工艺进行优化。采用扫描电镜(SEM)、激光粒度仪、高效液相色谱仪(HPLC)及生物测定对微胶囊的外观形貌、粒径、载药量、包封率、缓释性能、杀虫活性、非靶标生物安全性进行性能评价。[结果]各单因素对溴氰虫酰胺微胶囊制备均具有影响,影响主次依次为芯壁材质量比>油水相体积比>乳化剪切速度>聚乳酸浓度>磁力搅拌时间。正交试验优化制备工艺为芯壁材质量比1∶2、磁力搅拌时间4 h,聚乳酸质量浓度120 g/L、乳化剪切速度4500 r/min、油水相体积比1∶20。较优工艺制备的微胶囊载药量为(30.78±0.39)%、包封率为(92.34±1.16)%,微囊中位径为(16.89±0.21)μm、跨距为(1.13±0.02)μm。[结论]该方法制备的微胶囊表面光滑,光稳定性较好,持效期长,提高了对非靶标生物的安全性,可为药剂的使用提供借鉴。[Aims]Regarding the low utilization rate and high acute toxicity of cyantraniliprole to non-target organisms,high-performance cyantraniliprole polylactic acid microcapsules were prepared in order to provide reference for the use of this pesticide.[Methods]Cyantraniliprole polylactic acid microcapsules were prepared by solvent evaporation method with cyantraniliprole as the core material and polylactic acid as the wall material.The effects of different ratios of core and wall materials,wall material concentration,ratio of oil and water phase,emulsification shear speed,magnetic stirring speed,and stirring time on the drug loading,encapsulation efficiency,and particle size of cyantraniliprole microcapsules were investigated through single factor experiments.At the same time,the technical conditions for the preparation of microcapsules were optimized by the orthogonal experiment design L16(45)with drug loading and encapsulation efficiency as evaluation index.The appearance,particle size,drug loading,encapsulation efficiency,sustained release performance,insecticidal activity,and biological safety to non-target organisms of microcapsules were evaluated using SEM,laser particle size analyzer,HPLC and bioassay.[Results]All single factors had an impact on the preparation of cyantraniliprole microcapsules,with the main and secondary influencing factors being the mass ratio of core and wall material>ratio of oil and water phase>emulsification shear rate>polylactic acid concentration>magnetic stirring time.The orthogonal experiment optimized the preparation process as follows:the mass ratio of the core and wall material was 1∶2,the magnetic stirring time was 4 h,the concentration of polylactic acid was 120 g/L,the emulsification shear speed was 4500 r/min,and the volume ratio of oil and water phase was 1∶20.Under the optimal preparation process,the drug loading of microcapsules was(30.78±0.39)%,the encapsulation rate was(92.34±1.16)%,the median diameter was(16.89±0.21)μm,and the span was(1.13±0.02)μm.[Conclusions]

关 键 词：溴氰虫酰胺 聚乳酸 微胶囊 溶剂挥发法 性能评价 

分 类 号：TQ450.2[化学工程—农药化工]