Ultrasound-triggered biomimetic ultrashort peptide nanofiber hydrogels promote bone regeneration by modulating macrophage and the osteogenic immune microenvironment  

作　　者：Fan Zhang Mingchen Lv Siyuan Wang Mengyao Li Yu Wang Congjiao Hu Wei Hu Xuekui Wang Xiaogang Wang Zhiduo Liu Zhen Fan Jianzhong Du Yao Sun 

机构地区：[1]Department of Oral Implantology,Stomatological Hospital and Dental School of Tongji University,Shanghai Engineering Research Center of Tooth Restoration and Regeneration,200072,China [2]Department of Gynaecology and Obstetrics,Shanghai Fourth People’s Hospital,School of Medicine,Tongji University,Shanghai,200434,China [3]Department of Polymeric Materials,School of Materials Science and Engineering,Tongji University,201804,China [4]Department of Immunology and Microbiology,The Minister of Education Key Laboratory of Cell Death and Differentiation,Shanghai Institute of Immunology,Shanghai Jiao Tong University School of Medicine,China [5]Key Laboratory of Big Data-Based Precision Medicine,School of Engineering Medicine,Beihang University,China

出　　处：《Bioactive Materials》2024年第1期231-246,共16页生物活性材料（英文）

基　　金：financially supported by National Key R&D Program of China(2022YFA1103200);National Natural Science Foundation of China(81822012,82061130222,81771043,22075212,21925505,52222306,22075212);Shanghai Academic Leader of Science and Technology Innovation Action Plan(20XD1424000);Innovation Program of Shanghai Municipal Education Commission(2023ZKZD28);the Shanghai Experimental Animal Research Project of Science and Technology Innovation Action Plan(8191101676,201409006400);Shanghai International Scientific Collaboration Fund(21520710100).

摘　　要：The immune microenvironment plays a vital role in bone defect repair.To create an immune microenvironment that promotes osteogenesis,researchers are exploring ways to enhance the differentiation of M2-type macrophages.Functional peptides have been discovered to effectively improve this process,but they are limited by low efficiency and rapid degradation in vivo.To overcome these issues,peptide with both M2 regulatory and self-assembly modules was designed as a building block to construct an ultrasound-responsive nanofiber hydrogel.These nanofibers can be released from hydrogel in a time-dependent manner upon ultrasound stimulation,activating mitochondrial glycolytic metabolism and the tricarboxylic acid cycle,inhibiting reactive oxygen species production and enhancing M2 macrophage polarization.The hydrogel exhibits advanced therapeutic potential for bone regeneration by triggering M2 macrophages to secrete BMP-2 and IGF-I,accelerating the differentiation of bone marrow mesenchymal stem cells(BMSCs)into osteoblasts.Thus,modularly designed biomimetic ultrashort peptide nanofiber hydrogels provide a novel strategy to rebuild osteogenic immune microenvironments for bone repair.

关 键 词：PEPTIDE Nanofiber hydrogel Immune microenvironment Bone tissue regeneration 

分 类 号：O63[理学—高分子化学]