Mechanisms underlying the cell-matrixed nerve grafts repairing peripheral nerve defects  

作　　者：Shanshan Wang Hongkui Wang Panjian Lu Leilei Gong Xiaosong Gu Meiyuan Li 

机构地区：[1]Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education,Co-innovation Center of Neuroregeneration,NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products,Nantong University,Nantong,Jiangsu,226001,PR China [2]Department of Obstetrics and Gynecology,Affiliated Hospital of Nantong University,Nantong,Jiangsu,226001,PR China

出　　处：《Bioactive Materials》2024年第1期563-577,共15页生物活性材料（英文）

基　　金：supported by grants from the National Natural Science Foundation of China(Grant No.32130060,31730031 and 81901256);the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD).

摘　　要：Decellularized extracellular matrix(dECM),with its distinct biological properties,has gained significant attention as a natural biomaterial.Leveraging its potentials,we successfully developed a three-dimensional matrix-based oriented nerve graft by encapsulating a fibrous scaffold with multilayered conformationally intact and biologically active human bone marrow mesenchymal stem cell-derived decellularized extracellular matrix(hBMSC-dECM).Convincingly,the hBMSC-dECM group exhibited comparable functional recoveries to the autograft group by postoperative week 12.In the comprehensive analysis,the molecular regulations in the hBMSC-dECM group were more intricate and nuanced compared to the autograft group.Nevertheless,both groups displayed similar molecular regulatory processes in terms of vascularization and extracellular matrix.Notably,the hBMSC-dECM group demonstrated sustained high levels of regulation in axon and myelin regeneration at week 12,while the immunomodulation returned to the normal levels after peaking at week 2.Collectively,our findings illustrated the satisfactory construction of a cell-matrixed nerve graft that established a microenvironment conducive to nerve regeneration,and elucidated the distinct molecular regulation patterns and characteristics associated with different repair modes.

关 键 词：DECELLULARIZATION Extracellular matrix hBMSC Peripheral nerve regeneration Tissue engineering 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]