响应面法优化圆苞车前子多酚与总黄酮超声提取工艺及其抗氧化活性分析  

Optimization of ultrasonic extraction process of polyphenols and total flavonoids from Plantago ovata Forssk by response surface methodology and its antioxidant activity analysis

作　　者：排合尔丁·穆太力甫 木艾塔尔·努尔麦麦提 艾尼娃尔·艾克木 田树革[2] Paiheerding Mutailifu;Muaitaer Nuermaimaiti;Ainiwaer Aikemu;TIAN Shuge(College of Xinjiang Hetian,Hetian,Xinjiang 848000,China;College of Chinese Medicine,Xinjiang Medical University,Urumqi,Xinjiang 830017,China;Xinjiang Key Laboratory of Hetian Characteristic Chinese Medicine Research,Hetian,Xinjiang 848000,China)

机构地区：[1]新疆和田学院,新疆和田848000 [2]新疆医科大学中医学院,新疆乌鲁木齐830017 [3]新疆和田特色中医药研究重点实验室,新疆和田848000

出　　处：《湖南中医药大学学报》2024年第10期1801-1809,共9页Journal of Hunan University of Chinese Medicine

基　　金：新疆维吾尔自治区重大科技项目(2017A03005-2)。

摘　　要：目的本实验旨在优化圆苞车前子多酚和总黄酮提取工艺,探究圆苞车前子提取物抗氧化活性。方法采用单因素实验结合响应面Box-Behnken设计对圆苞车前子中多酚和总黄酮进行超声辅助提取工艺研究,以多酚及黄酮提取率为指标,利用响应面法得出最佳提取工艺。且通过DPPH、ABTS+自由基的清除能力和总还原能力测定评价其抗氧化能力。结果圆苞车前子多酚与总黄酮提取最佳工艺为:料液比1∶20,提取时间41 min,超声波功率420 W,乙醇体积分数88%。该条件下,多酚的提取量为4.79 mg/g,总黄酮的提取量为23.19 mg/g。抗氧化实验结果表明,圆苞车前子乙醇提取物在2.5~50μg/mL范围内,对DPPH、ABTS+自由基有较好的清除作用,IC50值分别为26.62μg/mL和9.40μg/mL;其总还原能力随着浓度的增加而增强,且始终高于同浓度维生素C的还原能力。结论该提取工艺稳定、可靠,可用于圆苞车前子多酚与总黄酮的提取,且圆苞车前子乙醇提取物具有较好的抗氧化活性。Objective To optimize the extraction process of polyphenols and total flavonoids from Plantago ovata Forssk,and explore the antioxidant activity of its extracts.Methods Single factor experiments combined with a response surface Box Behnken design were used to study the ultrasonic-assisted extraction process of polyphenols and total flavonoids from Plantago ovata Forssk.The optimal extraction process was determined using response surface methodology with the extraction rates of polyphenols and flavonoids serving as the main indicators.In addition,the antioxidant capacity of the extracts was evaluated by measuring the scavenging ability of DPPH and ABTS+free radicals and the total reducing power.Results The optimal extraction process for polyphenols and total flavonoids from Plantago ovata Forssk were as follows:solid-liquid ratio of 1∶20,extraction time of 41 min,ultrasonic power of 420 W,and ethanol volume fraction of 88%.Under these conditions,the extraction amounts of polyphenols and total flavonoids were 4.79 mg/g and 23.19 mg/g,respectively.The antioxidant experiment results showed that the ethanol extract of Plantago ovata Forssk had good scavenging effects on DPPH and ABTS+free radicals in the range of 2.5-50μg/mL,with the IC50 values of 26.62μg/mL and 9.40μg/mL,respectively.The total reducing power increased with increasing concentration and remains higher than that of vitamin C at the same concentration.Conclusion The extraction process is stable and reliable,and can be used for the extraction of polyphenols and total flavonoids from Plantago ovata Forssk.The ethanol extract of Plantago ovata Forssk exhibits good antioxidant activity.

关 键 词：圆苞车前子 超声波辅助提取 多酚 黄酮 响应面法 抗氧化 

分 类 号：R283.6[医药卫生—中药学] R285.5[医药卫生—中医学]