摩罗丹浓缩丸通过TNF/PI3K/AKT信号通路治疗慢性萎缩性胃炎  

Moluodan concentrated pill in the treatment of chronic atrophic gastritis through the TNF/PI3K/AKT signaling pathway

作　　者：陈锦[1] 何琳俐 高颖 张宽[1,2] CHEN Jin;He Linli;GAO Ying;ZHANG Kuan(Department of Pharmacy,The Second Affiliated Hospital of Fujian University of Traditional Chinese Medicine,Fuzhou 350003,China;Key Laboratory for Traditional Chinese Medicine Preparations in Medical Institutions of Fujian Province,Fuzhou 350003,China)

机构地区：[1]福建中医药大学附属第二人民医院药学部,福州350003 [2]福建省医疗机构中药制剂重点实验室,福州350003

出　　处：《数理医药学杂志》2024年第11期823-830,共8页Journal of Mathematical Medicine

摘　　要：目的探究摩罗丹浓缩丸(Moluodan concentrated pill,MLD)治疗慢性萎缩性胃炎(chronic atrophic gastritis,CAG)潜在的分子机制。方法利用中药系统药理学数据库与分析平台(Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform,TCMSP)、中医药综合数据库(Traditional Chinese Medicine Integrated Database,TCMID)、中医药整合药理学研究平台(Integrative Pharmacologybased Research Platform of Traditional Chinese Medicine,TCMIP)和中药免疫肿瘤学数据库(Traditional Chinese Medicine on Immuno-Oncology,TCMIO)获取MLD的化合物和化合物相关靶点(compound-related target,CRT);DisGeNET和GeneCards数据库获取CAG相关靶点基因(CAG related target gene,CAG-RTG);采用Cytoscape 3.7.2软件构建MLD化合物-CRT网络,并将CRT和CAG-RTG取交集获取MLD相关疾病靶点(MLD-related disease target,MLD-RDT);利用STRING数据库构建MLD-RDT的蛋白质-蛋白质相互作用(protein-protein interaction,PPI)网络,并进行拓扑结构分析,筛选重要靶点。采用基因本体论(Gene Ontology,GO)富集分析与京都基因和基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)信号通路富集分析探索MLD治疗CAG的主要分子机制。结果MLD中共有259个活性分子,获得961个靶基因,其中179个可能与CAG相关。PPI网络显示,AKT1、TNF、IL-6、TP53、IL-1β等是MLD治疗CAG的关键靶点。富集分析显示,MLD治疗CAG的关键通路为PI3K-AKT信号通路和TNF信号通路。结论MLD可能通过介导TNF/PI3K/AKT信号通路治疗CAG。Objective To explore the potential molecular mechanism of Moluodan concentrated pill(MLD)in the treatment of chronic atrophic gastritis(CAG).Methods The Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP),Traditional Chinese Medicine Integrated Database(TCMID),Integrative Pharmacologybased Research Platform of Traditional Chinese Medicine(TCMIP),and Traditional Chinese Medicine on Immuno-Oncology(TCMIO)were used to obtain the compounds of MLD and compound-related targets(CRTs).DisGeNET and GeneCards databases were used to obtain CAG related target genes(CAG-RTGs).The MLD compound-CRT network was constructed using Cytoscape 3.7.2 software,and the intersection of CRTs and CAG-RTGs was taken to obtain MLD-related disease targets(MLD-RDTs).The STRING database was used to construct the protein-protein interaction(PPI)network of MLD-RDTs and perform topological structure analysis to screen for important targets.Gene Ontology(GO)enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)signaling pathway enrichment analysis were used to explore the main molecular mechanism of MLD in the treatment of CAG.Results A total of 259 active molecules in MLD obtained 961 target genes,of which 179 may be related to CAG.The PPI network showed that AKT1,TNF,IL-6,TP53,IL-1β,etc.were the key targets of MLD in the treatment of CAG.Enrichment analysis showed that the key pathways of MLD in the treatment of CAG were the PI3K-AKT signaling pathway and the TNF signaling pathway.Conclusion MLD may treat CAG by mediating the TNF/PI3K/AKT signaling pathway.

关 键 词：摩罗丹浓缩丸 慢性萎缩性胃炎 TNF/PI3K/AKT信号通路 分子机制 

分 类 号：R573.3[医药卫生—消化系统]