早期推拿对坐骨神经损伤模型大鼠腓肠肌生物力学参数及Myod、Myog、Mef2c表达的影响  

Effects of Early Tuina on the Biomechanical Parameters of the Gastrocnemius Muscle and the Expression of Myod,Myog,and Mef2c in a Rat Model of Sciatic Nerve Injury

作　　者：韩珊 吕桃桃 田野 冯智伟 魏芳远 秦丽娜 吴俊德 覃颖 谢丹丹 石慧敏 李笑涵 吴凡[2] 张琳[2] Han Shan;Lu Taotao;Tian Ye;Feng Zhiwei;Wei Fangyuan;Qin Lina;Wu Junde;Qin Ying;Xie Dandan;Shi Huimin;Li Xiaohan;Wu Fan;Zhang Lin(The Third Affiliated Hospital of Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]北京中医药大学第三附属医院,北京100029 [2]北京联合大学,北京100101

出　　处：《中国中医急症》2024年第11期1914-1919,共6页Journal of Emergency in Traditional Chinese Medicine

基　　金：国家重点研发计划“常见多发病防治研究”重点专项(2022YFC2503900);北京中医药大学新教师启动基金项目(2023-JYB-XJSJJ-058)。

摘　　要：目的探究早期推拿对坐骨神经损伤(SNI)模型大鼠腓肠肌肌肉状态及Myod、Myog、Mef2c表达的影响。方法将30只SPF级雄性SD大鼠随机分为假手术组、模型组和推拿O、T、F组,每组各6只。假手术组仅暴露右侧坐骨神经,不做夹持;模型组和推拿组建立坐骨神经夹持损伤模型。推拿O组在造模后第1天开始干预,总共干预7次;推拿T组在造模后第3天开始干预,总共干预5次;推拿F组在造模后第5天开始干预,总共干预3次。干预方法为每天以4 N的按摩力度,揉、拨、点法刺激大鼠术侧阳陵泉、殷门、委中穴,每法每穴1 min,每天1次。上述手法采用按摩推拿手法模拟仪进行操作。假手术组和模型组每天抓取,不作推拿干预。使用MyotonPRO数字化肌肉功能评估系统评估患侧腓肠肌振动频率(F)、对数衰减值(D)及动态刚度(S)3个生物机械力学特性参数;HE染色观察患侧腓肠肌肌纤维的形态学变化;Real-Time PCR法检测Myod mRNA、Myog mRNA、Mef2c mRNA表达水平;Western blotting法检测腓肠肌中Myod、Myog蛋白表达。结果治疗后,与假手术组相比,模型组、推拿F组的动态频率、对数衰减、动态刚度及推拿T组的动态刚度升高,差异具有统计学意义(P<0.05);与模型组相比,推拿O、T组振动频率、对数衰减、动态刚度及推拿F组动态刚度降低,差异具有统计学意义(P<0.05)。HE染色显示,假手术组大鼠患侧腓肠肌肌纤维排列致密、紧实、有序,模型组肌纤维紊乱、松散,部分组织断裂,炎细胞浸润;推拿组O、T组肌纤维排列等状态均接近假手术组,推拿F组肌纤维排列相对有序,存在部分组织断裂。PCR结果显示,与假手术组相比,模型组和推拿各组Myod mRNA、Myog mRNA、Mef2c mRNA表达升高,差异具有统计学意义(P<0.05);与模型组相比,推拿各组Myod mRNA、Myog mRNA、Mef2c mRNA表达升高,且推拿O组高于推拿T组,推拿T组高于推拿F组,差异具有统计学意义(P<0.0Objective:To explore the effects of early Tuina on the muscle condition of the gastrocnemius and the expression of Myod,Myog,and Mef2c in a rat model of sciatic nerve injury(SNI).Methods:30 SPF-grade male SD rats were randomly divided into:sham group(n=6),model group(n=6),Tuina O group(n=6),Tuina T group(n=6),and Tuina F group(n=6).The sham group had the right sciatic nerve exposed without clamping;the model group and Tuina groups established the sciatic nerve clamping injury model.The Tuina O group received intervention from the first day after modeling,with a total of seven sessions;the Tuina T group received intervention from the third day after modeling,with a total of five sessions;the Tuina F group received intervention from the fifth day after modeling,with a total of three sessions.The intervention involved massaging the ipsilateral Yanglingquan,Yinmen,and Weizhong acupoints with 4 N of force using rubbing,pinching,and pressing techniques,for 1 minute per acupoint,once daily.These manipulations were performed using a Tuina manipulation simulator.The sham and model groups were handled daily without Tuina intervention.The MyotonPRO system was used to assess three biomechanical properties of the gastrocnemius muscle:vibration frequency(F),logarithmic decrement(D),and dynamic stiffness(S).HE staining was performed to observe morphological changes in the muscle fibers of the gastrocnemius muscle;Real-Time PCR was used to detect the expression levels of Myod mRNA,Myog mRNA,and Mef2c mRNA;and Western blotting analysis was used to detect the expression of Myod and Myog proteins in the gastrocnemius muscle.Results:After treatment,compared with the sham group,the model group and Tuina F group showed significant increases in dynamic frequency,logarithmic decrement,and dynamic stiffness,while the Tuina T group showed a significant increase in dynamic stiffness(P<0.05).Compared with the Model group,the Tuina O and T groups showed significant decreases in vibration frequency,logarithmic decrement,and dynamic stffness,w

关 键 词：坐骨神经损伤 推拿 腓肠肌 生物力学参数 大鼠 

分 类 号：R745.4[医药卫生—神经病学与精神病学]