湖南一株猪非典型瘟病毒的鉴定及其NS5B和E2基因序列分析  

Identification of a strain of atypical porcine pestivirus in Hunan Province and analysis of its NS5B and E2 gene sequence

作　　者：柳泰 胡兴业 毛婉 谢邵波 尹德明[1] 余兴龙[1] LIU Tai;HU Xingye;MAO Wan;XIE Shaobo;YIN Deming;YU Xinglong(College of Veterinary Medicine,Hunan Agricultural University,Changsha,Hunan 410128,China)

机构地区：[1]湖南农业大学动物医学院,湖南长沙410128

出　　处：《湖南农业大学学报（自然科学版）》2024年第5期98-103,共6页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：湖南省重点领域研发计划项目(2022NK2049)。

摘　　要：随机挑选湖南某规模化猪场出现“抖抖病”的新生仔猪,采集血清样本,用可扩增猪非典型瘟病毒(APPV)的NS5B基因和E2基因引物进行RT-PCR鉴定,对PCR扩增产物测序。随后利用MegAlign软件分析NS5B和E2基因序列的同源性,预测E2蛋白部分结构,并使用MEGA 5.0,采用邻接法构建NS5B和E2基因的遗传进化树。结果表明,发病仔猪为猪非典型瘟病毒(APPV)感染阳性。鉴定毒株HN0626与GenBank中84株参考毒株NS5B和E2基因序列的同源性分别为81.68%~98.95%、81.14%~99.33%,通过预测E2蛋白部分结构,推导其潜在的B细胞抗原表位。系统发育分析显示,HN0626毒株属于基因1型,是全球猪群中的主要流行毒株,其E2、NS5B基因序列在GenBank中的登录号分别为OR936135、OR936136。We collected serum samples from the randomly selected“dancing pigs”from a large-scale pig farm in Hunan Province,and identified them by RT-PCR using primers for the NS5B and E2 genes that can amplify the atypical porcine pestivirus(APPV),and then sequenced the PCR amplified products.Subsequently,the homology of NS5B and E2 gene sequences was analyzed using MegAlign software to predict part of the structure of the E2 protein,and the genetic evolution tree of NS5B and E2 genes was constructed using the neighbor-joining method,using MEGA 5.0.It indicated that the affected piglets were positive for the atypical porcine pestivirus(APPV)infection.The homology of the gene sequences of strain HN0626 with those of 84 reference strains of NS5B and E2 in GenBank was identified as 81.68%-98.95%and 81.14%-99.33%,respectively,and its potential B-cell antigenic epitopes were successfully deduced.Phylogenetic analysis showed that strain HN0626 belongs to genotype 1,which is the main prevalent strain in the global swine population,and its E2 and NS5B gene sequences were accessed in GenBank with the accession numbers OR936135 and OR936136,respectively.

关 键 词：猪非典型瘟病毒 NS5B基因 E2基因 抗原表位预测 湖南 

分 类 号：S858.280.1[农业科学—临床兽医学]