常规病理苏木素——伊红染色室间质量评价结果分析  

Analysis of inter-laboratory quality evaluation results of routine pathological hematoxylin-eosin staining

作　　者：薛晓伟[1] 王德田[1] 凌庆[1] 李亚腾 周炜洵[1] Xue Xiaowei;Wang Detian;Ling Qing;Li Yateng;Zhou Weixun(Department of Pathology,Peking Union Medical College Hospital,Peking Union Medical College,Chinese Academy of Medical Sciences,Beijing 100730,China)

机构地区：[1]中国医学科学院北京协和医学院北京协和医院病理科,北京100730

出　　处：《中国医学装备》2024年第11期137-140,共4页China Medical Equipment

基　　金：中央高水平医院临床科研专项(2022-PUMCH-C-018)。

摘　　要：目的:通过分析中国医学装备协会病理装备分会主办的常规技术苏木素-伊红(HE)染色室间质量评价结果,梳理HE染色技术经验和问题,探讨医疗机构HE染色水平和室间质量控制管理能力的提升路径。方法:收集2022年和2023年中国医学装备协会病理装备分会主办的全国常规病理HE染色室间质量评价结果资料,对比两年中参与HE染色室间质量评价的测评单位等级、组织类型及测评成绩。结果:2022年参加HE染色室间质量评价且成绩有效的测评单位共165家,2023年为156家;HE染色组织类型包括子宫组织、胃肠组织、肺组织和乳腺组织。2023年参加测评单位染色合格率90.38%(141/156)高于2022年82.42%(136/165),差异有统计学意义(x2=4.30,P<0.05)。2023年参评的三级医院染色合格率与2022年比较,差异无统计学意义(P>0.05);二级医院及非公医疗机构的合格率分别为100%和92.37%,均高于2022年,差异有统计学意义(x2=3.91、5.93,P<0.05)。2023年测评的子宫组织、胃肠组织、肺组织和乳腺组织室间质量评价合格率分别为92.31%(144/156)、91.03%(142/156)、91.03%(142/156)和91.67%(143/156),均高于2022年测评结果,子宫组织和胃肠组织两年测评比较差异有统计学意义(x2=6.33、5.93,P<0.05),肺组织和乳腺组织两年测评比较差异无统计学意义(P>0.05)。结论:HE染色室间质量评价能够提升常规病理HE染色质量和病理技术人员水平,减少因单位性质及标本类型不同造成的染色差异。Objective:By summarizing and analyzing the results of the inter-laboratory quality evaluation of hematoxylin-eosin(HE)staining organized by the Pathological Equipment Branch of Chinese Medical Equipment Association,to timely identify problems and summarize experience,and to improve the level of HE staining and the ability of inter-laboratory quality control management in medical institutions.Methods:The results of the national routine pathology HE staining inter-laboratory quality evaluation sponsored by the Pathology Equipment Branch of the China Medical Equipment Association in 2022 and 2023 were collected,and the grades,tissue types and evaluation results of the evaluation units participating in the inter-laboratory quality evaluation of HE staining were statistically analyzed.Results:In 2022,a total of 165 evaluation units participated in the inter-laboratory quality evaluation of HE staining with valid results,and 156 in 2023.HE stained tissue types included uterine tissue,gastrointestinal tissue,lung tissue,and breast tissue.The qualified rates of HE staining of the participating units in 2023 were 82.42%(136/165),which was higher than that of 90.38%(141/156)in 2022,the difference was statistically significant(x2=4.30,P<0.05).There was no statistically significant difference in the staining pass rate of the tertiary hospitals participating in the evaluation in 2023 compared with that in 2022(P>0.05).The qualified rates of secondary hospitals and nonpublic medical institutions were 100%and 92.37%,respectively,which were higher than those in 2022,the difference was statistically significant(x2=3.91,5.93,P<0.05).The qualified rates of inter-laboratory quality evaluation of uterine tissue,gastrointestinal tissue,lung tissue and breast tissue evaluated in 2023 were 92.31%(144/156),91.03%(142/156),91.03%(142/156)and 91.67%(143/156),respectively,which were higher than those in 2022,the differences between uterine tissue and gastrointestinal tissue in two-year evaluation were statistically significant(x2=6.33,5.93,

关 键 词：苏木素-伊红染色 室间质量评价 数据分析 合格率 

分 类 号：R361[医药卫生—病理学]