茄子果皮转录组SSR位点分析及标记开发  

Analysis of Simple Sequence Repeat(SSR)Loci and Marker Development in Eggplant(Solanum melongena L.)Pericarp Transcriptome

作　　者：王宏[1] 聂智星 杨舒桓 邵志勇 王同林 郑积荣[1] WANG Hong;NIE Zhixing;YANG Shuheng;SHAO Zhiyong;WANG Tonglin;ZHENG Jirong(Vegetable Research Institute,Hangzhou Academy of Agricultural Sciences,Hangzhou,Zhejiang 310024;College of Plant Science and Technology,Huazhong Agricultural University,Wuhan,Hubei 430070)

机构地区：[1]杭州市农业科学研究院蔬菜研究所,浙江杭州310024 [2]华中农业大学植物科学技术学院,湖北武汉430070

出　　处：《北方园艺》2024年第21期1-8,共8页Northern Horticulture

基　　金：杭州市市院合作资助项目(杭农[2021]58号);杭州市农业科学院科技创新与示范推广基金资助项目(2022HNCT-02);浙江省农业(蔬菜)新品种选育重大科技专项资助项目(2021C02065);杭州市农业与社会发展科研引导资助项目(20220919Y158)。

摘　　要：以茄子(Solanum melongena L.)果皮样品转录组的36568条转录本为试材,采用MISA(MicroSAtellite)软件进行SSR位点搜索,研究SSR位点的分布频率和特征,同时利用Primer 3.0软件对检索出的SSR位点设计引物,验证引物有效性,以期为茄子的种质鉴定、亲缘关系分析、分子标记辅助育种及遗传图谱构建等提供参考依据。结果表明:在转录本中共检测出9775个SSR位点,分布于6289条转录本上,平均5.49 kb存在1个SSR位点,发生频率26.73%。SSR位点特征分析显示,单核苷酸为优势重复基元类型,占SSR位点总数的47.67%;A/T基元是数量最多的重复基元,占总数的46.13%;97.91%的重复基元的重复次数在5~25,92.21%的SSR重复序列长度分布在10~30 bp。对所有SSR位点进行引物设计,共获得6619对EST-SSR引物。随机选择27对EST-SSR引物在12个茄子自交系中进行PCR扩增,引物有效性为92.59%,多态率为33.33%。Taking 36568transcripts from eggplant(Solanum melongena L.)pericarp samples as the test materials,SSR locus search was conducted using the MISA(MicroSAtellite)software.The distribution frequency and characteristics of SSR loci were studied,and primers for the retrieved SSR loci were designed using Primer 3.0software,while verifying the effectiveness of the primers,in order to provide reference for germplasm identification,phylogenetic analysis,molecular marker assisted breeding,and genetic map construction of eggplants.The results showed that a total of 9775SSR loci were detected,distributed on 6289transcripts.The frequency of the SSR loci was 26.73%,with an average distance of 5.49kb.SSR loci characteristics analysis revealed that mononucleotide was the dominant repeat motif type,accounting for 47.67%of the total number of SSRs.The A/T motif was the most numerous repeat motif type,accounting for 46.13%of the total.97.91%of the repeated motifs had a repetition frequency between 5-25,and 92.21%of the SSR sequence length was mainly distributed between 10-30bp.After primer design for all SSR loci,a total of 6619pairs of EST-SSR primers were obtained.27pairs of EST-SSR primers were randomly selected for PCR amplification in 12 eggplant inbred lines.The validity of the primer was 92.59%,and the polymorphism rate was 33.33%.

关 键 词：茄子(Solanum melongena L.) 果皮转录组 SSR位点 标记开发 

分 类 号：S641.1[农业科学—蔬菜学]