和厚朴酚对红托竹荪腐烂病菌抑菌活性的影响  

Effects of Honokiol on the Antibacterial Activity of Trichoderma koningii

作　　者：张超 文庭池[1,2,3] 曾祥羽 郭明[4] 谯利军 ZHANG Chao;WEN Tingchi;ZENG Xiangyu;GUO Ming;QIAO Lijun(School of Pharmacy,Guizhou University,Guiyang,Guizhou 550025;State Key Laboratory of Green Pesticide,Guizhou University,Guiyang,Guizhou 550025;Engineering Research Center of Southwest Bio-Pharmaceutical Resources,Ministry of Education,Guizhou University,Guiyang,Guizhou 550025;Guizhou Jinchangdashan Biotechnology Co.Ltd.,Nayong,Guizhou 553300;Guizhou Health Vocational College,Tongren,Guizhou 554300)

机构地区：[1]贵州大学药学院,贵州贵阳550025 [2]贵州大学绿色农药全国重点实验室,贵州贵阳550025 [3]贵州大学西南药用生物资源教育部工程研究中心,贵州贵阳550025 [4]贵州金蟾大山生物科技有限公司,贵州纳雍553300 [5]贵州健康职业学院,贵州铜仁554300

出　　处：《北方园艺》2024年第21期92-98,共7页Northern Horticulture

基　　金：铜仁市科技基金资助项目([2022]0-50);贵州省科技重大专项资助项目([2019]3005-5)。

摘　　要：以红托竹荪腐烂病菌(Trichoderma koningii)为试材,采用不同浓度的和厚朴酚对红托竹荪腐烂病菌进行处理,研究和厚朴酚对红托竹荪腐烂病菌菌丝生长、形态结构和抗氧化酶活性的影响,以期为红托竹荪腐烂病的绿色防控提供参考依据。结果表明:和厚朴酚对红托竹荪腐烂病菌具有显著的抑菌活性,其EC_(50)值为6.252μg·mL^(-1),经过和厚朴酚处理后的红托竹荪腐烂病菌菌丝形态发生明显变化,其菌丝表面呈现出不规则的皱缩与扭曲,出现明显的质壁分离现象,细胞器损伤,细胞内出现大面积空白。且生理系统紊乱,超氧化物歧化酶(SOD)活性升高,过氧化氢酶(CAT)、过氧化物酶(POD)活性降低,导致活性氧在体内过量积累,引起细胞损伤,导致菌丝生长受到抑制。综上,和厚朴酚可显著抑制红托竹荪腐烂病菌的生长,未来可作为红托竹荪腐烂病绿色防控的药物。Trichoderma koningii was used as the test material,different concentrations of honokiol were used to treat T.koningii to study the effects of honokiol on mycelia growth,morphological structure and antioxidant enzyme activity of T.koningii,in order to provide reference for green prevention and control of Phallus rubrovolvatus rot disease.The results showed that honokiol had significant antibacterial activity against T.koningii,and its EC_(50)value was 6.252μg·mL^(-1).After treatment with honokiol,the mycelial morphology of T.koningii showed significant changes.The surface of the mycelium showed irregular shrinkage and distortion,there was obvious plasmic wall separation,organelle damage,and there was a large area of blank space in the cell.In addition,the physiological system was disturbed,superoxide dismutase(SOD)activity increased,catalase(CAT)and peroxidase(POD)activity decreased,resulted in excessive reactive oxygen species accumulation,cell damage,and mycelial growth was inhibited.In conclusion,honokiol could significantly inhibit the growth of T.koningii,and could be used as green prevention and control drugs for Phallus rubrovolvatus rot disease in the future.

关 键 词：和厚朴酚 红托竹荪腐烂病 抑菌活性 抗氧化酶活性 

分 类 号：S646[农业科学—蔬菜学]