Alkali burn injury model of meibomian gland dysfunction in mice  

作　　者：Yong Li Ya-Qiong Yang Yong Lin Ke Yan Yu-Fei Lyu Zhao-Qiang Zhang Cai-Hong Huang Jiao-Yue Hu Zu-Guo Liu 

机构地区：[1]Xiamen University Affiliated Xiamen Eye Center,Eye Institute of Xiamen University,School of Medicine,Xiamen University,Xiamen 3611002,Fujian Province,China [2]The Second Affiliated Hospital,Hengyang Medical School,University of South China,Hengyang 421000,Hunan Province,China [3]Fujian Provincial Key Laboratory of Ophthalmology and Visual Science,Fujian Engineering and Research Center of Eye Regenerative Medicine,Xiamen 3611002,Fujian Province,China [4]Department of Traditional Chinese Medicine,School of Medicine,Xiamen University,Xiamen 361100,Fujian Province,China [5]Department of Ophthalmology,the First Affiliated Hospital of University of South China,Hengyang 421001,Hunan Province,China

出　　处：《International Journal of Ophthalmology(English edition)》2024年第12期2158-2166,共9页国际眼科杂志（英文版）

基　　金：Supported by the National Natural Science Foundation of China(No.82271054,No.U20A20363).

摘　　要：AIM:To establish a stable,short-time,low-cost and reliable murine model of meibomian gland dysfunction(MGD).METHODS:A filter paper sheet soaked in 1.0 mol/L sodium hydroxide(NaOH)solution was used to touch the eyelid margin of C57BL/6J mice for 10s to establish the model.The other eye was left untreated as a control group.Eyelid margin morphological changes and the meibomian glands(MGs)were observed by slit lamp microscopy on days 5 and 10 post-burn.Hematoxylin-eosin(HE)staining and Oil red O staining were adopted in detecting the changes in MGs morphology and lipid deposition.Real-time polymerase chain reaction,Western blot,immunofluorescence staining and immunohistochemical staining were used to detect interleukin(IL)-6,IL-1β,IL-18,tumor necroses factor(TNF)-α,interferon(IFN)-γ,nicotinamide adenine dinucleotide phosphate(NADPH)oxidase 4(NOX4),3-nitroturosine(3-NT),4-hydroxynonenal(4-HNE)and cytokeratin 10(K10)expression changes in MGs.RESULTS:MGs showed plugging of orifice,glandular deficiency,abnormal acinar morphology,ductal dilatation,and lipid deposition after alkali burn.The expressions of IL-6,IL-18,IL-1β,IFN-γ,and TNF-αindicators of inflammation and oxidative stress in MGs tissues were significantly increased.Abnormal keratinization increased in the MG duct.CONCLUSION:A murine model of MGD is established by alkali burn of the eyelid margin that matches the clinical presentation of MGD providing a stable,short-time,lowcost,and reliable MGD model.The new method suggests efficient avenues for future research.

关 键 词：alkali burn meibomian gland dysfunction animal model oxidative stress 

分 类 号：R777.1[医药卫生—眼科]