Biocompatibility of light responsive materials prepared for accommodative intraocular lenses manufacturing  

作　　者：Chen-Zi Liu Ming-Hao Sun Wei Dai Lan-Cao Hao Si-Quan Zhu 

机构地区：[1]Department of Ophthalmology,Beijing Anzhen Hospital,Capital Medical University,Beijing 100029,China [2]Department of Ophthalmology,Xiamen Ophthalmology Center,Xiamen University,Xiamen 361004,Fujian Province,China

出　　处：《International Journal of Ophthalmology(English edition)》2024年第12期2167-2176,共10页国际眼科杂志（英文版）

基　　金：Supported by the National Natural Science Foundation of China(No.52073181,No.52273134).

摘　　要：AIM:To investigate the biocompatibility and bacterial adhesion properties of light responsive materials(LRM)and analyze the feasibility and biosafety of employing LRM in the preparation of accommodative intraocular lenses(AIOLs).METHODS:Employing fundamental experimental research techniques,LRM with human lens epithelial cells(hLECs)and human retinal pigment epithelium cells(ARPE-19 cells)were co-cultured.Commercially available intraocular lenses(IOLs)were used as controls to perform cell counting kit-8(CCK-8),cell staining under varying light intensities,cell adhesion and bacterial adhesion experiments.RESULTS:LRM exhibited a stronger inhibitory effect on the proliferation of ARPE19 cells than commercially available IOLs when co-cultured with the undiluted extract for 96h(P<0.05).Under other culturing conditions,the effects on the proliferation of hLECs and ARPE-19 cells were not significantly different between the two materials.Under the influence of light irradiation at intensities of 200 and 300 mW/cm^(2),LRM demonstrated a markedly higher inhibitory effect on the survival of hLECs compared to commercially available IOLs(P<0.0001).They also showed a stronger suppressive effect on the survival rate of ARPE-19 cells,with significant differences observed at 200 mW/cm^(2)(P<0.001)and extremely significant differences at 300 mW/cm^(2)(P<0.0001).Additionally,compared to commercially available IOLs,LRM had a higher number of cells adhering to their surface(P<0.05),as well as a significantly greater number of adherent bacterium(P<0.0001).CONCLUSION:LRM,characterized by their excellent non-contact tunable deformability and low cytotoxicity to ocular tissues,show considerable potential for use in the fabrication of AIOLs.These materials demonstrate strong cell adhesion;however,during photothermal conversion processes involving shape deformation under various light intensities,the resultant temperature rise may harm surrounding cells.These factors suggest that while the material plays a positive role in reducing the inc

关 键 词：light responsive materials accommodative intraocular lens BIOCOMPATIBILITY bacterial adhesion 

分 类 号：R779.6[医药卫生—眼科]