Modeling monocular form deprivation in rabbits using a simulated-cataract intraocular lens  

作　　者：Si-Yi Gu Li-Ming Xu Wei-Jie Sun Li-Li Liang Lei Lin Han Zou Jing-Yuan Xu Yu Zheng Yuan-Yuan Li Yin-Ying Zhao Ping-Jun Chang Yun-E Zhao 

机构地区：[1]Eye Hospital and School of Ophthalmology and Optometry,Wenzhou Medical University,Wenzhou 325003,Zhejiang Province,China [2]The State Key Laboratory of Optometry,Ophthalmology and Vision Science,Wenzhou 325003,Zhejiang Province,China [3]National Center for Clinical and Medical Research,Wenzhou 325003,Zhejiang Province,China [4]Eye Hospital of Wenzhou Medical University at Hangzhou,Hangzhou 310000,Zhejiang Province,China

出　　处：《International Journal of Ophthalmology(English edition)》2024年第12期2177-2184,共8页国际眼科杂志（英文版）

基　　金：Supported by National Natural Science Foundation of China(No.81870680).

摘　　要：AIM:To establish an animal model of form deprivation amblyopia based on a simulated cataract intraocular lens(IOLs).METHODS:Poly(dimethyl siloxane)-SiO_(2) thin films(PSF)with different degrees of opacity as IOL materials were prepared.The light transmission of the PSF-IOL was measured,and its in vitro biosafety was determined by cell counting kit(CCK)-8 assay using the HLEC-B3 cell line and ARPE-19 cell line.Subsequently,the in vivo safety was determined by implanting the PSF-IOL with 10%wt SiO_(2) into the right eyes of New Zealand white rabbits(PSF-IOL group),and compared with two control groups:contralateral comparison group and normal control(NC)group(Contralateral comparison group:the fellow eye;NC group:a group of binocular normal rabbits without intervention).The flash visual-evoked potentials(F-VEPs)were measured to verify amblyopia.RESULTS:PSFs containing 0,2%,and 10%wt SiO_(2) were successfully constructed.The 0 SiO_(2) PSF was transparent,while the 10%wt SiO_(2) PSF was completely opaque.It was found that PSF did not induce unwanted cytotoxicity in HLECs and ARPE19 cells in vitro.In vitro,PSF-IOL with 10%wt SiO_(2) was also non-toxic,and no significant inflammation or structural changes occurred after four weeks of PSF-IOL implantation.Finally,our IOL-simulated congenital cataract rabbit detected by F-VEPs suggested tentative amblyopia.CONCLUSION:A PSF-IOL that mimics cataracts is created.A novel form deprivation model is created by the IOL-simulated congenital cataract rabbit.It can be developed fast and stable and holds great potential for future study.

关 键 词：monocular deprivation form deprivation intraocular lens congenital cataract AMBLYOPIA 

分 类 号：R779.6[医药卫生—眼科]