大黄酚对EGF诱导的甲状腺乳头状癌TPC-1细胞增殖、迁移、侵袭和EMT抑制作用的影响  

Effect of chrysophanol on proliferation,migration,invasion and EMT induced by EGF in papillary thyroid carcinoma TPC-1 cells

作　　者：李忠祥 王晓欧 张萌 何巍[1] LI Zhong-xiang;WANG Xiao-ou;ZHANG Meng;HE Wei(Department of Pharmacy,The First Affiliated Hospital of China Medical University,Shenyang 110001,China;Department of General Surgery,Central Hospital Affiliated to Shenyang Medical College,Shenyang 110024,China)

机构地区：[1]中国医科大学附属第一医院药学部,辽宁沈阳110001 [2]沈阳医学院附属中心医院普外科,辽宁沈阳110024

出　　处：《解剖科学进展》2024年第4期432-435,共4页Progress of Anatomical Sciences

基　　金：沈阳医学院硕士研究生科技创新基金(Y20220531)。

摘　　要：目的探究大黄酚对EGF诱导的甲状腺乳头状癌TPC-1细胞增殖、迁移、侵袭和EMT的抑制作用,并探讨其可能机制。方法TPC-1细胞分为对照组、EGF组、大黄酚低剂量组、大黄酚中剂量组和大黄酚高剂量组。采用CCK-8法测定细胞增殖活性,Transwell实验检测细胞迁移和侵袭能力,Western blot测定细胞EMT相关蛋白N-cadherin和Vimentin以及Claudin 1蛋白表达及EGFR磷酸化水平。结果EGF处理增加TPC-1细胞增殖、迁移和侵袭能力,上调细胞中N-cadherin、Vimentin、p-EGFR和Claudin 1蛋白表达水平。大黄酚处理剂量依赖的抑制TPC-1细胞增殖、迁移和侵袭能力,降低细胞中N-cadherin、Vimentin、p-EGFR和Claudin 1蛋白表达水平。结论大黄酚抑制了EGF诱导的PTC细胞增殖、迁移、侵袭和EMT,其机制可能与抑制EGFR激活并下调Claudin 1表达有关。Objective To explore the inhibitory effect of chrysophanol on EGF-induced proliferation,migration,invasion and EMT of thyroid papillary carcinoma TPC-1 cells,and to explore its possible mechanism.Methods TPC-1 cells were divided into control group,EGF group,low-dose,medium-dose and high-dose chrysophanol group.Cell proliferation activity was detected by CCK-8 assay,cell migration and invasion ability was detected by Transwell assay,and the expressions of EMT-related proteins N-cadherin,Vimentin,Claudin 1 and EGFR phosphorylation levels were determined by Western blot.Results EGF treatment increased the proliferation,migration and invasion ability of TPC-1 cells,and up-regulated the expression levels of N-cadherin,Vimentin,p-EGFR and Claudin 1 proteins in TPC-1 cells.Chrysophanol treatment inhibited the proliferation,migration and invasion of TPC-1 cells in a dose-dependent manner,and decreased the expression levels of N-cadherin,Vimentin,p-EGFR and Claudin 1 proteins in the cells.Conclusion Chrysophanol inhibited EGF-induced proliferation,migration,invasion and EMT of PTC cells,and the mechanism may be related to inhibiting EGFR activation and down-regulating Claudin 1 expression.

关 键 词：大黄酚 甲状腺乳头状癌 TPC-1细胞 EGF Claudin 1 

分 类 号：R736.1[医药卫生—肿瘤]