消退素E1对炎症微环境下根尖牙乳头细胞的影响  

Effect of resolvin E1 on the apical papilla cells in inflammatory microenvironment

作　　者：潘萍 刘玉三[1] 李言君[1] 崔彩云 PAN Ping;LIU Yusan;LI Yanjun;CUI Caiyun(Department of Oral Medicine,Binzhou Medical University Hospital,Binzhou 256603,Shandong,P.R.China)

机构地区：[1]滨州医学院附属医院口腔内科,山东滨州256603

出　　处：《滨州医学院学报》2024年第5期355-359,370,共6页Journal of Binzhou Medical University

基　　金：山东省自然科学基金(ZR2023MH039)。

摘　　要：目的 探讨消退素E1(resolvin E1,RvE1)对脂多糖(Lipopolysaccharide, LPS)诱导的炎症微环境下根尖牙乳头细胞(apical papilla cells, APCs)成牙本质方向分化及细胞自噬的影响。方法 将人APCs分为对照组、LPS组和LPS RvE1组,应用MTT检测细胞增殖情况,应用realtime-PCR检测成牙本质方向分化相关基因表达情况,应用茜素红染色及氯化十六烷基吡啶半定量分析检测APCs矿化结节形成情况,应用Western blot检测APCs自噬相关蛋白表达情况。结果 与对照组比较,LPS组的APCs增殖受到抑制,成牙本质方向分化及矿化结节形成减弱,细胞自噬增强(P<0.05)。与LPS组比较,LPS RvE1组的APCs增殖、成牙本质方向分化及矿化结节形成均增强,细胞自噬减弱(P<0.05)。结论 RvE1促进炎症微环境下APCs成牙本质方向分化和降低自噬。Objective To investigate the effects of resolvin E1(RvE1)on the odontogenic differentiation and autophagy of APCs in the inflammatory microenvironment induced by lipolyaccharide(LPS).Methods Human APCs were divided into the control group,the LPS group and the LPS RvE1 group.MTT was used to detect cell proliferation,realtime-PCR was used to detect the expression of genes related to odontogenic differentiation,alizarin red staining and cetylpyridine chloride semi-quantitative analysis were used to detect the formation of mineralized nodules,and Western blot was used to detect the expression of autophagy related proteins.Results Compared with those in the control group,the inhibition of cell proliferation,odontogenic differentiation and the formation of mineralized nodules were weakened in the LPS group,and autophagy was enhanced(P<0.05).Compared with those in the LPS group,cell proliferation,odontogenic differentiation and mineralization nodule formation were enhanced in the LPS RvE1 group,and autophagy was weakened(P<0.05).Conclusion RvE1 promotes odontogenic differentiation of APCs and decreases autophagy in inflammatory environment.

关 键 词：消退素E1 脂多糖 根尖牙乳头细胞 成牙本质分化 自噬 

分 类 号：R781.8[医药卫生—口腔医学]