Effect of chitin-architected spatiotemporal three-dimensional culture microenvironments on human umbilical cord-derived mesenchymal stem cells  

作　　者：Shuoji Zhu Junfeng Xuan Yunchao Shentu Katsuhiko Kida Masaki Kobayashi Wei Wang Minoru Ono Dehua Chang 

机构地区：[1]Department of Cardiac Surgery,University of Tokyo,Tokyo,113-8655,Japan [2]Department of Cell Therapy in Regenerative Medicine,University of Tokyo Hospital,Tokyo,113-8655,Japan [3]Nissan Chemical Corporation,Tokyo,103-0027,Japan [4]Winhealth Pharma,999077,Hong Kong

出　　处：《Bioactive Materials》2024年第5期291-305,共15页生物活性材料（英文）

摘　　要：Mesenchymal stem cell(MSC)transplantation has been explored for the clinical treatment of various diseases.However,the current two-dimensional(2D)culture method lacks a natural spatial microenvironment in vitro.This limitation restricts the stable establishment and adaptive maintenance of MSC stemness.Using natural polymers with biocompatibility for constructing stereoscopic MSC microenvironments may have significant application potential.This study used chitin-based nanoscaffolds to establish a novel MSC three-dimensional(3D)culture.We compared 2D and 3D cultured human umbilical cord-derived MSCs(UCMSCs),including dif-ferentiation assays,cell markers,proliferation,and angiogenesis.When UCMSCs are in 3D culture,they can differentiate into bone,cartilage,and fat.In 3D culture condition,cell proliferation is enhanced,accompanied by an elevation in the secretion of paracrine factors,including vascular endothelial growth factor(VEGF),hepa-tocyte growth factor(HGF),Interleukin-6(IL-6),and Interleukin-8(IL-8)by UCMSCs.Additionally,a 3D culture environment promotes angiogenesis and duct formation with HUVECs(Human Umbilical Vein Endothelial Cells),showing greater luminal area,total length,and branching points of tubule formation than a 2D culture.MSCs cultured in a 3D environment exhibit enhanced undifferentiated,as well as higher cell activity,making them a promising candidate for regenerative medicine and therapeutic applications.

关 键 词：Cellhesion®chitin nanoscaffolds Mesenchymal stem cells STEMNESS 3D culture 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]