Development of ovalbumin implants with different spatial configurations for treatment of peripheral nerve injury  

作　　者：Tiantian Zheng Hongxia Gao Yaqiong Liu Shaolan Sun Wenchao Guan Linliang Wu Yumin Yang Guicai Li 

机构地区：[1]Key Laboratory of Neuroregeneration,Co-innovation Center of Neuroregeneration,Nantong University,Nantong,226001,China [2]National Engineering Research Center for Biomaterials,Sichuan University,Chengdu,610065,China [3]The People’s Hospital of Rugao,Affiliated Hospital of Nantong University,226599,Nantong,China

出　　处：《Bioactive Materials》2024年第5期401-415,共15页生物活性材料（英文）

基　　金：the financial support of the National Natural Science Foundation of China(32171352);Special Funds for Provincial Science and Technology Programs(Key R&D Program for Social Development)of Jiangsu Province(BE2023743);Open Research Fund of State Key Laboratory of Advance Technology for Materials Synthesis and Processing(Wuhan University of Technology,2023-KF-18);Open Research Fund of State Key Laboratory of Bioelectronics,Southeast University(2023-K05);Opening Project of State Key Laboratory of Polymer Materials Engineering(Sichuan University,Sklpme2022-4-01);226 High-level Talent Training Project(2nd level,2022 II-276).

摘　　要：Peripheral nerve injury(PNI)seriously affects the health and life of patients,and is an urgent clinical problem that needs to be resolved.Nerve implants prepared from various biomaterials have played a positive role in PNI,but the effect should be further improved and thus new biomaterials is urgently needed.Ovalbumin(OVA)contains a variety of bioactive components,low immunogenicity,tolerance,antimicrobial activity,non-toxicity and biodegradability,and has the ability to promote wound healing,cell growth and antimicrobial properties.However,there are few studies on the application of OVA in neural tissue engineering.In this study,OVA implants with different spatial structures(membrane,fiber,and lyophilized scaffolds)were constructed by casting,electrospinning,and freeze-drying methods,respectively.The results showed that the OVA implants had excellent physicochemical properties and were biocompatible without significant toxicity,and can promote vascularization,show good histocompatibility,without excessive inflammatory response and immunogenicity.The in vitro results showed that OVA implants could promote the proliferation and migration of Schwann cells,while the in vivo results confirmed that OVA implants(the E5/70%and 20 kV 20μL/min groups)could effectively regulate the growth of blood vessels,reduce the inflammatory response and promote the repair of subcutaneous nerve injury.Further on,the high-throughput sequencing results showed that the OVA implants up-regulated differential expression of genes related to biological processes such as tumor necrosis factor-α(TNF-α),phosphatidylinositide 3-kinases/protein kinase B(PI3K-Akt)signaling pathway,axon guidance,cellular adhesion junctions,and nerve regeneration in Schwann cells.The present study is expected to provide new design concepts and theoretical accumulation for the development of a new generation of nerve regeneration implantable biomaterials.

关 键 词：OVALBUMIN Casting method ELECTROSPINNING Freeze drying Nerve scaffold Peripheral nerve injury 

分 类 号：R318[医药卫生—生物医学工程]