Type II collagen scaffolds repair critical-sized osteochondral defects under induced conditions of osteoarthritis in rat knee joints via inhibiting TGF-β-Smad1/5/8 signaling pathway  

作　　者：Xu Hu Min Jin Kang Sun Zhen Zhang Zhonglian Wu Junli Shi Peilai Liu Hang Yao Dong-An Wang 

机构地区：[1]Department of Biomedical Engineering,City University of Hong Kong,83 Tat Chee Avenue,Kowloon,Hong Kong [2]Karolinska Institutet Ming Wai Lau Centre for Reparative Medicine,HKSTP,Sha Tin,Hong Kong [3]Department of Orthopedics,Qilu Hospital of Shandong University,107 Wenhua Xilu,Jinan,PR China [4]School of Chemistry and Chemical Engineering,Yangzhou University,Yangzhou,PR China [5]Shenzhen Research Institute,City University of Hong Kong,Shenzhen,PR China

出　　处：《Bioactive Materials》2024年第5期416-428,共13页生物活性材料（英文）

基　　金：supported by General Research Fund,Research Grants Council,University Grants Committee,Hong Kong SAR(CityU 11205520 to Dong-An Wang);National Natural Science Foundation of China(NSFC51973180 to Dong-An Wang);Grant from Karolinska Institutet Ming Wai Lau Centre of Reparative Medicine (to Dong-An Wang);and, Grants from City University of Hong Kong (7020028, 7005949, 9231412, 9231486 to Dong-An Wang);Young scientists lifting project of Jiangsu Province, China (TJ-2022-072 to Hang Yao).

摘　　要：The bidirectional relationship between osteochondral defects(OCD)and osteoarthritis(OA),with each condition exacerbating the other,makes OCD regeneration in the presence of OA challenging.Type II collagen(Col2)is important in OCD regeneration and the management of OA,but its potential applications in cartilage tissue engineering are significantly limited.This study investigated the regeneration capacity of Col2 scaffolds in critical-sized OCDs under surgically induced OA conditions and explored the underlying mechanisms that promoted OCD regeneration.Furthermore,the repair potential of Col2 scaffolds was validated in over critical-sized OCD models.After 90 days or 150 days since scaffold implantation,complete healing was observed histologically in critical-sized OCD,evidenced by the excellent integration with surrounding native tissues.The newly formed tissue biochemically resembled adjacent natural tissue and exhibited comparable biomechanical properties.The regenerated OA tissue demonstrated lower expression of genes associated with cartilage degradation than native OA tissue but comparable expression of genes related to osteochondral anabolism compared with normal tissue.Additionally,transcriptome and proteome analysis revealed the hindrance of TGF-β-Smad1/5/8 in regenerated OA tissue.In conclusion,the engrafting of Col2 scaffolds led to the successful regeneration of critical-sized OCDs under surgically induced OA conditions by inhibiting the TGF-β-Smad1/5/8 signaling pathway.

关 键 词：OSTEOARTHRITIS Osteochondral defect Tissue engineering Type II collagen 

分 类 号：R318[医药卫生—生物医学工程]