Bioinspired engineering ADSC nanovesicles thermosensitive hydrogel enhance autophagy of dermal papilla cells for androgenetic alopecia treatment  

作　　者：Jiachao Xiong Zhixiao Liu Lingling Jia Yulin Sun Rong Guo Tingting Xi Zihan Li Minjuan Wu Hua Jiang Yufei Li 

机构地区：[1]Department of Plastic Surgery,Shanghai East Hospital,School of Medicine,Tongji University,Shanghai,200120,China [2]Medical College,Tongji University,Shanghai,200331,China [3]Department of Histology and Embryology,Naval Medical University,Shanghai,200433,China [4]St Hugh’s College,University of Oxford,OX26LE,United Kingdom

出　　处：《Bioactive Materials》2024年第6期112-125,共14页生物活性材料（英文）

基　　金：supported by the Featured Clinical Discipline Project of Shanghai Pudong Fund(Grant No.PWYts2021-07);the East Hospital Affiliated to Tongji University Introduced Talent Research Startup Fund(Grant No.DFRC2019008);the National Natural Science Foundation of China(Grant No.32071186).

摘　　要：Androgenic alopecia(AGA)is a highly prevalent form of non-scarring alopecia but lacks effective treatments.Stem cell exosomes have similar repair effects to stem cells,suffer from the drawbacks of high cost and low yield yet.Cell-derived nanovesicles acquired through mechanical extrusion exhibit favorable biomimetic properties similar to exosomes,enabling them to efficiently encapsulate substantial quantities of therapeutic proteins.In this study,we observed that JAM-A,an adhesion protein,resulted in a significantly increased the adhesion and resilience of dermal papilla cells to form snap structures against damage caused by dihydrotestosterone and macrophages,thereby facilitating the process of hair regrowth in cases of AGA.Consequently,adipose-derived stem cells were modified to overexpress JAM-A to produce engineered JAM-A overexpressing nanovesicles(JAM-A^(OE)@NV).The incorporation of JAM-A^(OE)@NV into a thermosensitive hydrogel matrix(JAM-A^(OE)@NV Gel)to effectively addresses the limitations associated with the short half-life of JAM-A^(OE)@NV,and resulted in the achievement of a sustained-release profile for JAM-A^(OE)@NV.The physicochemical characteristics of the JAM-A^(OE)@NV Gel were analyzed and assessed for its efficacy in promoting hair regrowth in vivo and vitro.The JAM-A^(OE)@NV Gel,thus,presents a novel therapeutic approach and theoretical framework for promoting the treatment of low cell adhesion diseases similar to AGA.

关 键 词：Androgenic alopecia NANOVESICLES JAM-A AUTOPHAGY HYDROGEL 

分 类 号：R318[医药卫生—生物医学工程]