Cu(II)-baicalein enhance paracrine effect and regenerative function of stem cells in patients with diabetes  

作　　者：Kaijing Liu Ruihao Li Shusen Wang Xue Fu Ni Zhu Xiaoyu Liang Huiyang Li Xiaoli Wang Le Wang Yongjun Li Jianwu Dai Jing Yang 

机构地区：[1]Key Laboratory of Advanced Medical Materials and Devices,Institute of Biomedical Engineering,Tianjin Institutes of Health Science,Chinese Academy of Medical Science&Peking Union Medical College,Tianjin,300192,China [2]Tianjin Medical Health Research Institute,Tianjin,300192,China [3]Organ Transplant Center,Tianjin First Central Hospital,Nankai University,Tianjin,China [4]Tianjin Clinical Research Center for Organ Transplantation,Tianjin,China [5]Department of Vascular Surgery,Beijing Hospital,National Center of Gerontology,Institute of Geriatric Medicine,Chinese Academy of Medical Science&Peking Union Medical College,Beijing,100730,China [6]Graduate School of Peking Union Medical College,Beijing,100730,China

出　　处：《Bioactive Materials》2024年第6期455-473,共19页生物活性材料（英文）

基　　金：National Natural Science Foundation of China(82072080);CAMS Innovation Fund for Medical Sciences(2023-I2M-2-008,2022-I2M-3-002,2021-12M-1-058);supported by Special Program for High-Tech Leader&Teams of Tianjin Government;Tianjin innovation Promotion Plan Key Innovation Team of Immunreactive Biomaterials.

摘　　要：The development of engineered or modified autologous stem cells is an effective strategy to improve the efficacy of stem cell therapy.In this study,the stemness and functionality of adipose stem cells derived from type 1 diabetic donors(T1DM-ASC)were enhanced by treatment with Cu(II)-baicalein microflowers(Cu-MON).After treatment with Cu-MON,T1DM-ASC showed enhanced expression of the genes involved in the cytokine-cytokine receptor interaction pathway and increased cytokine secretion.Among the top 13 differentially expressed genes between T1DM-ASC and Cu-MON-treated T1DM-ASC(CMTA),some genes were also expressed in HUVEC,Myoblast,Myofibroblast,and Vascular Smooth Muscle cells,inferring the common role of these cell types.In vivo experiments showed that CMTA had the same therapeutic effect as adipose-derived stem cells from non-diabetic donors(ND-ASC)at a 15%cell dose,greatly reducing the treatment cost.Taken together,these findings suggest that Cu-MON promoted angiogenesis by promoting the stemness and functionality of T1DM-ASC and influencing multiple overall repair processes,including paracrine effects.

关 键 词：ANGIOGENESIS Adipose stem cells Type 1 diabetes mellitus Paracrine effect Regenerative function 

分 类 号：R587.1[医药卫生—内分泌]