A ROS-responsive hydrogel incorporated with dental follicle stem cell-derived small extracellular vesicles promotes dental pulp repair by ameliorating oxidative stress  

作　　者：Mengjie Li Jun Tian Kangkang Yu He Liu Xiaoqi Yu Nan Wang Qimei Gong Kun Li Ya Shen Xi Wei 

机构地区：[1]Hospital of Stomatology,Guanghua School of Stomatology,Sun Yat-Sen University,Guangzhou,China [2]Guangdong Provincial Key Laboratory of Stomatology,Guangzhou,China [3]Key Laboratory of Bio-resources and Eco-environment,Ministry of Education,College of Life Sciences,Sichuan University,Chengdu,China [4]Division of Endodontics,Department of Oral Biological and Medical Sciences,The University of British Columbia,Vancouver,British Columbia,Canada [5]Key Laboratory of Green Chemistry and Technology,Ministry of Education,College of Chemistry,Sichuan University,Chengdu,China [6]Department of Pharmaceutical Engineering,College of Food and Bioengineering,Xihua University,Chengdu,China

出　　处：《Bioactive Materials》2024年第6期524-540,共17页生物活性材料（英文）

基　　金：supported by the National Natural Science Foundation of China(No.82370943,82201037 and 81970925);the Young Elite Scientists Sponsorship Program by Guangzhou(QT-2023-030);Fundamental Research Funds for the Central Universities,Sun Yat-sen University(23qnpy157).

摘　　要：Pulpitis,an inflammatory disease of dental pulp tissues,ultimately results in the loss of pulp defense properties.Existing clinical modalities cannot effectively promote inflamed pulp repair.Oxidative stress is a major obstacle inhibiting pulp repair.Due to their powerful antioxidative capacity,mesenchymal stem cell-derived small extracellular vesicles(MSC-sEVs)exhibit potential for treating oxidative stress-related disorders.However,whether MSC-sEVs shield dental pulp tissues from oxidative damage is largely unknown.Here,we showed that dental follicle stem cell-derived sEVs(DFSC-sEVs)have antioxidative and prohealing effects on a rat LPS-induced pulpitis model by enhancing the survival,proliferation and odontogenesis of H_(2)O_(2)-injured dental pulp stem cells(DPSCs).Additionally,DFSC-sEVs restored the oxidative/antioxidative balance in DPSC mitochondria and had comparable effects on ameliorating mitochondrial dysfunction with the mitochondrion-targeted antioxidant Mito-Tempo.To improve the efficacy of DFSC-sEVs,we fabricated an intelligent and injectable hydrogel to release DFSC-sEVs by combining sodium alginate(SA)and the ROS sensor RhB-AC.The newly formed SA-RhB hydrogel efficiently encapsulates DFSC-sEVs and exhibits controlled release of DFSC-sEVs in a HClO/ClO^(-)concentration-dependent manner,providing a synergistic antioxidant effect with DFSC-sEVs.These results suggest that DFSC-sEVs-loaded SA-RhB is a promising minimally invasive treatment for pulpitis by enhancing tissue repair in the pulp wound microenvironment.

关 键 词：Small extracellular vesicle Dental follicle stem cell Pulpitis Oxidative stress ROS-Responsive hydrogel 

分 类 号：R78[医药卫生—口腔医学] TQ427.26[医药卫生—临床医学]