Hydrophobic surface induced pro-metastatic cancer cells for in vitro extravasation models  

作　　者：Minseok Lee Seunggyu Kim Sun Young Lee Jin Gyeong Son Joonha Park Seonghyeon Park Jemin Yeun Tae Geol Lee Sung Gap Im Jessie S.Jeon 

机构地区：[1]Department of Chemical and Biomolecular Engineering,Korea Advanced Institute of Science and Technology,Daehak-ro 291,Yuseong-gu,Daejeon,34141,Republic of Korea [2]Department of Mechanical Engineering,Korea Advanced Institute of Science and Technology,Daehak-ro 291,Yuseong-gu,Daejeon,34141,Republic of Korea [3]Bioimaging Team,Safety Measurement Institute,Korea Research Institute of Standards and Science(KRISS),Gajeong-ro 267,Yuseong-gu,Daejeon,34113,Republic of Korea [4]KAIST Institute for the NanoCentury(KINC),Korea Advanced Institute of Science and Technology,Daehak-ro 291,Yuseong-gu,Daejeon,34141,Republic of Korea

出　　处：《Bioactive Materials》2024年第4期401-413,共13页生物活性材料（英文）

基　　金：supported by the National Research Foundation of Korea(NRF)grant funded by the Ministry of Science and ICT(MSIT)of Korea(No.2021R1A2B5B03001416,No.2020R1A2C1100471,No.2020R1A5A8018367);the BK21 FOUR Program of the NRF grant funded by the Ministry of Education(MOE)of Korea.

摘　　要：In vitro vascularized cancer models utilizing microfluidics have emerged as a promising tool for mechanism study and drug screening.However,the lack of consideration and preparation methods for cancer cellular sources that are capable of adequately replicating the metastatic features of circulating tumor cells contributed to low relevancy with in vivo experimental results.Here,we show that the properties of cancer cellular sources have a considerable impact on the validity of the in vitro metastasis model.Notably,with a hydrophobic surface,we can create highly metastatic spheroids equipped with aggressive invasion,endothelium adhesion capabilities,and activated metabolic features.Combining these metastatic spheroids with the well-constructed microfluidic-based extravasation model,we validate that these metastatic spheroids exhibited a distinct extravasation response to epidermal growth factor(EGF)and normal human lung fibroblasts compared to the 2D cultured cancer cells,which is consistent with the previously reported results of in vivo experiments.Furthermore,the applicability of the developed model as a therapeutic screening platform for cancer extravasation is validated through profiling and inhibition of cytokines.We believe this model incorporating hydrophobic surface-cultured 3D cancer cells provides reliable experimental data in a clear and concise manner,bridging the gap between the conventional in vitro models and in vivo experiments.

关 键 词：Initiated chemical vapor deposition Cancer spheroid Cancer extravasation Organ-on-a-chip In vitro disease model 

分 类 号：R73[医药卫生—肿瘤] R318[医药卫生—临床医学]