Synthesis,Cytotoxicity,Apoptosis and Cell Cycle Arrest of a Ruthenium(II)-Substituted Keggin Polyoxotungstate  

钌多取代Keggin-型多钨酸盐的合成型多钨酸盐的合成、细胞毒性、细胞凋亡和细胞周期研究

作　　者：JIA Shifang HAO Xiuli WENYanzhen SHI Shaoqi ZHANG Yan 贾士芳;郝秀丽;温艳珍;史少奇;张燕(太原科技大学化学工程与技术学院,山西太原030024)

机构地区：[1]College of Chemical Engineering and Technology,Taiyuan University of Science and Technology,Taiyuan 030024,Shanxi,China

出　　处：《Wuhan University Journal of Natural Sciences》2024年第5期461-470,共10页武汉大学学报（自然科学英文版）

基　　金：Supported by the National Natural Science Foundation of China (21701120);the Science and Technology Innovation Project of Colleges and Universities in Shanxi Province (2020L0334);the Innovation and Entrepreneurship Training Program for College Students in Shanxi Province(20240778)。

摘　　要：The ruthenium multi-substituted polyoxotungstate,K_(7)[Si W_(9)O_(37)Ru_(4)(H_(2)O)_(3)Cl_(3)]·15H_(2)O(S1),was synthesized by a conventional aqueous solution containing the trilacunary Keggin-anionsβ-Na_(9)HSi W_(9)O_(34)·12H_(2)O(S2)and Ru Cl_(3)·n H_(2)O(S3).Compound S1 was charac‐terized by elemental analysis,energy-dispersive X-ray spectroscopy(EDS),thermogravimetric analysis(TG),infrared spectroscopy(IR),uliraviolet visible absorption spectroscopy(UV/Vis)and X-ray photoelectron spectroscopy(XPS).The cytotoxicitycy of S1 was tested in C33A(human cervical cancer),DLD-1(human colon cancer),Hep G2(human liver cancer)and human normal embryonic lung fibroblasts cell(MRC-5).And the viability of these treated cells was evaluated by MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bro‐mide)assay.To explore the mode of cell death induced by S1,morphological study of DNA damage and apoptosis assays were conducted.These analyses revealed that S1 exerted its cytotoxic effect in a dose-dependent manner,primarily triggering apoptotic cell death.Cell cycle analysis by flow cytometry indicated that compound S1 caused cell cycle arrest and accumulated cells in S phase.本文以三缺位Keggin-型阴离子β-Na_(9)HSi W_(9)O_(34)·12H_(2)O (S2)和Ru Cl_(3)·n H_(2)O (S3)为原料,在水溶液中合成了一个钌多取代多钨酸盐,其化学式为:K_(7)[Si W_(9)O_(37)Ru_(4)(H_(2)O)_(3)Cl_(3)]·15H_(2)O (S1)。通过元素分析、能谱、热重、红外、紫外和X-射线光电子能谱对化合物S1进行了表征。通过MTT法测试了化合物S1对C33A、DLD-1、Hep G2三种肿瘤细胞和人正常胚肺成纤维细胞MRC-5的细胞毒性。通过观察细胞形态及流式细胞仪考察了肿瘤细胞的死亡方式。实验结果表明化合物S1诱导肿瘤细胞凋亡而非坏死,并且细胞存活率与S1的浓度呈梯度关系。最后通过流式细胞仪分析细胞周期的变化,结果显示化合物S1使细胞周期停留在S期。

关 键 词：ruthenium multi-substituted polyoxometalate CYTOTOXICITY cell apoptosis cell cycle arrest 

分 类 号：O614[理学—无机化学]