液相色谱-串联质谱法在中药材真菌毒素检测中的应用  

Application of Liquid Chromatography-tandem Mass Spectrometry inDetection of Mycotoxins in Chinese Medicinal Materials

作　　者：焦洁[1,2] 张环[1,2] 李玉芳[1,2] 黄铮[1,2] JIAO Jie;ZHANG Huan;LI Yufang;HUANG Zheng(Institute of Animal Husbandry,Pasture and Green Agriculture,Gansu Academy of Agricultural Sciences,Lanzhou Gansu 730070,China;Institute of Agricultural Quality Standards and Testing Technology,Gansu Academy of Agricultural Sciences,Lanzhou Gansu 730070,China)

机构地区：[1]甘肃省农业科学院畜草与绿色农业研究所,甘肃兰州730070 [2]甘肃省农业科学院农业质量标准与检测技术研究所,甘肃兰州730070

出　　处：《寒旱农业科学》2024年第11期1073-1078,共6页Journal of Cold-Arid Agricultural Sciences

基　　金：甘肃省农业科学院农业科技创新专项(2020GAAS15);甘肃省自然科学基金计划项目(22JR5RA764)。

摘　　要：中药材作为传统医学的重要组成部分,在种植、运输、储藏中极易被污染产生真菌毒素,严重影响了药材质量和药效。液相色谱-串联质谱法(LC-MS/MS)可准确快速检测中药材中的真菌毒素种类和残留水平,并获得多种真菌毒素的含量信息,提高检测效率的同时减少了样品处理时间,降低操作复杂性。本文阐述了LC-MS/MS法检测中药材真菌毒素的种类、样品前处理方法及其流动相、检出限、加标回收率、定量方法等,对其在中药材真菌毒素检测中的应用前景进行了展望。As an important part of traditional medicine,Chinese medicinal materials are easy to be polluted and produce mycotoxins during planting,transportation and storage,which seriously affect the quality and efficacy of medicinal materials.Liquid chromatography-tandem mass spectrometry(LC-MS/MS)can accurately and rapidly detect the types and residual levels of mycotoxins in Chinese medicinal materials,and obtain the content information of various mycotoxins,improve the detection efficiency,reduce the sample processing time,and reduce the complexity of operation.In this paper,the types,sample pretreatment methods,mobile phase,detection limit,recovery rate and quantitative methods of LC-MS/MS for the detection of mycotoxins in Chinese medicinal materials were described,and the application prospect of LC-MS/MS in the detection of mycotoxins in Chinese medicinal materials was prospected.

关 键 词：中药材 真菌毒素 LC-MS/MS 前处理方法 检出限 加标回收率 

分 类 号：R284[医药卫生—中药学]