香菇多糖和冷冻平衡时间对猪精液冷冻保存效果的影响  

Effects of lentinan and equilibration time on boar semen cryopreservation

作　　者：王芳[1,2] 亓美玉[1] 马红[1] 刘娣 WANG Fang;QI Meiyu;MA Hong;LIU Di(Institute of Animal Husbandry,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China;Postdoctoral Workstation of Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China)

机构地区：[1]黑龙江省农业科学院畜牧研究所,哈尔滨150086 [2]黑龙江省农业科学院博士后科研工作站,哈尔滨150086

出　　处：《黑龙江畜牧兽医》2024年第21期51-54,126,共5页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家生猪技术创新中心先导项目(NCTIP-XD1C16);国家自然科学基金项目(U20A2052);黑龙江省省属科研院所科研业务费项目(CZKYF2023-1-C004);黑龙江省农业科学院创新跨越工程科技攻关项目(2021YYYF021);黑龙江省农业科学院博士后科研启动金项目。

摘　　要：为了研究香菇多糖和冷冻平衡时间对猪精液冷冻保存效果的影响,试验在公猪精液的冷冻稀释液中添加0.6 mg/mL的香菇多糖,冷冻平衡0,30,60,90,120,150 min后,分别采用观察法、异硫氰荧光棒标记的花生凝集素(FITC-PNA)染色法、低渗肿胀法和吖啶橙染色法检测冻后猪精子的活率、顶体完整率、质膜完整率和DNA完整性。结果表明:冻后猪精子的活率和顶体完整率随着平衡时间的延长呈先升高后降低的趋势,在平衡90分钟时达到最高,分别为45.19%、55.74%;冻后猪精子的质膜完整率在平衡30,60,120,150 min之间差异不显著(P>0.05),在平衡90分钟时最高,达到51.47%,显著高于其他平衡时间质膜完整率(P<0.05);冻后猪精子DNA完整率在平衡30,60,90,120 min之间差别不显著(P>0.05),其中平衡90 min时最高,为44.56%。说明冷冻稀释液中添加0.6 mg/mL香菇多糖并平衡90 min能显著提高猪精液的冷冻保存效果。In order to explore the effects of lentinan and equilibration time on boar semen cryopreservation,0.6 mg/mL lentinan was added into the frozen diluent of boar semen and frozen for 0,30,60,90,120,150 min,then the sperm motility,acrosome integrity,membrane integrity and DNA integrity after thawing were measured by observation with microscopy,FITC-PNA staining,HOST and acridine orange staining,respectively.The results showed that sperm motility and acrosome integrity increased first and then decreased,and reached the highest values at 90 min(45.19%,55.74%,respectively).The membrane integrity showed no significant difference at equilibrium 30,60,120,150 min groups(P>0.05)and reached the highest value at 90 min(51.47%),which was significantly higher than that at other equilibrium times(P<0.05).There was no significant difference in DNA integrity among 30,60,90,and 120 min groups after freezing(P>0.05),and the highest DNA integrity was 44.56%at 90 min.These results indicated that adding 0.6 mg/mL lentinan in the frozen diluent and equilibration for 90 min could significantly improve the cryopreservation effect of boar semen.

关 键 词：猪 精液 冷冻保存 香菇多糖 平衡时间 

分 类 号：S828[农业科学—畜牧学] S814[农业科学—畜牧兽医]