多花黄精炮制前后化学成分差异的UPLC-Q-Exactive Orbitrap MS分析  

Differences in Chemical Composition of Polygonatum cyrtonema Rhizome Before and After Processing Based on UPLC-Q-Exactive Orbitrap MS Analysis

作　　者：任萃姣 丁杨飞 李靖季 历帅帅 李鹏飞 张伟 赵宏苏 许凤清 金传山 刘军玲 REN Cuijiao;DING Yangfei;LI Jingji;LI Shuaishuai;LIPengfei;ZHANG Wei;ZHAO Hongsu;XU Fengqing;JIN Chuanshan;LIU Junling(School of Pharmacy,Traditional Chinese Medicine(TCM)Processing Inheritance Base of National Administration of TCM,Ministry of Education-Anhui Joint Collaborative Innovation Center for Quality Improvement of Anhui Genuine Chinese Medicinal Materials,Anhui Key Laboratory of New Technology for Manufacturing TCM Decoction Pieces,Anhui University of Chinese Medicine,Hefei 230012,China;Key Laboratory of Quality Research and Evaluation of TCM of National Medical Products Administration,Anhui Institute for Food and Drug Control,Hefei 230051,China)

机构地区：[1]安徽中医药大学药学院,国家中医药管理局中药炮制传承基地,省部共建安徽道地中药材品质提升协同创新中心,中药饮片制造新技术安徽省重点实验室,合肥230012 [2]安徽省食品药品检验研究院,国家药品监督管理局中药质量研究与评价重点实验室,合肥230051

出　　处：《中国实验方剂学杂志》2024年第24期183-189,共7页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：安徽省高校与合肥综合性国家科学中心协同创新项目(GXXT-2022-066)。

摘　　要：目的:基于超高效液相色谱-四极杆-静电场轨道阱高分辨质谱法(UPLC-Q-Exactive Orbitrap MS)分析多花黄精生品与酒制品化学成分差异。方法:采用UPLC-Q-Exactive Orbitrap MS对多花黄精炮制前后的化学成分进行分析,原始数据处理后提取有效响应离子,结合多元统计分析并根据变量重要性投影(VIP)值>1、P<0.05、差异倍数(FC)>2或FC<0.5等条件筛选多花黄精炮制前后的差异化合物,基于保留时间、准分子离子、碎片离子等信息并结合对照品及文献进行成分鉴定,通过聚类热分析确定显著性差异化合物并进行相对定量分析,以明确多花黄精炮制前后主要成分的变化规律。结果:共鉴定出多花黄精生品与酒制品差异成分72个,包括生物碱类化合物15个、有机酸类化合物12个、氨基酸类化合物12个、黄酮类化合物6个、糖类化合物4个、其他类化合物23个。共有显著性差异成分18个,其中L-苹果酸、乳酸、天师酸等13个化合物在酒制后含量呈上升趋势;反式-3-吲哚丙烯酸、L-精氨酸、D-色氨酸等5个化合物在酒制后含量呈下降趋势。结论:多花黄精炮制前后化学成分存在明显差异,主要为有机酸类、糖类、氨基酸类、黄酮类、生物碱类化合物,该研究可为黄精炮制机制的深入研究奠定基础。Objective:Ultra performance liquid chromatography-quadrupole-electrostatic field orbitrap high resolution mass spectrometry(UPLC-Q-Exactive Orbitrap MS)was used to analyze the differences in chemical components between raw products and wine-processed products of Polygonatum cyrtonema rhizome.Method:UPLC-Q-Exactive Orbitrap MS was used to analyze the chemical compositions of P.cyrtonema rhizome before and after processing,and the effective response ions were extracted after raw data processing,and the differential compounds before and after processing were screened combined with multivariate statistical analysis and according to the conditions of variable importance in the projection(VIP)value>1,P<0.05,fold change(FC)>2 or FC<0.5,based on the retention time,quasi-molecular ions,fragment ions and other information,the components were identified in combination with the control products and the literature,and the significant difference compounds were identified by clustering thermal analysis and relative quantitative analyzed,in order to clarify the change rule of the main components in P.cyrtonema rhizome before and after processing.Result:A total of 72 differential constituents between raw products and wine-processed products were identified,including 15 alkaloids,12 organic acids,12 amino acids,6 flavonoids,4 saccharides and 23 others.There were a total of 18 significantly different components,among which 13 compounds,including L-malic acid,lactic acid and 9,12,13-trihydroxy-10-octadecenoic acid,showed an increasing trend in content after wine processing,5 compounds such as trans-3-indoleacrylic acid,L-arginine,D-tryptophan,showed a decreasing trend after processing.Conclusion:The chemical components of P.cyrtonema rhizome are significantly different before and after processing,mainly organic acids,saccharides,amino acids,flavonoids and alkaloids,which can lay the foundation for the in-depth study of the processing mechanism of Polygonati Rhizoma.

关 键 词：黄精 酒制多花黄精 超高效液相色谱-四极杆-静电场轨道阱高分辨质谱法(UPLC-Q-Exactive Orbitrap MS) 相对定量分析 化学成分差异 中药炮制 

分 类 号：R22[医药卫生—中医基础理论] R28[医药卫生—中医学] R943.1[理学—分析化学] O657[理学—化学]