单细胞水平解析人第三磨牙牙囊组织免疫微环境特征  

Single-cell level analysis of the immune microenvironment characteristics of dental follicle tissues from the human third molars

作　　者：刘佳宁 张小慧 曹元 刘露 雷啸 田炯义 何珺希 金钫 隋秉东 LIU Jianing;ZHANG Xiaohui;CAO Yuan;LIU Lu;LEI Xiao;TIAN Jiongyi;HE Junxi;JIN Fang;SUI Bingdong(State Key Laboratory of Oral&Maxillofacial Reconstruction and Regeneration,National Clinical Research Center for Oral Diseases,Shaanxi International Joint Research Center for Oral Diseases,Center for Tissue Engineering,Xi'an 710032;Department of Orthodontics,The Third Hospital Affiliated of Air Force Military Medical University,China,Xi'an 710032)

机构地区：[1]口颌系统重建与再生全国重点实验室,国家口腔疾病临床医学研究中心,陕西省口腔疾病国际联合研究中心,空军军医大学第三附属医院组织工程中心,西安710032 [2]口颌系统重建与再生全国重点实验室,国家口腔疾病临床医学研究中心,陕西省口腔疾病国际联合研究中心,空军军医大学第三附属医院口腔正畸科,西安710032

出　　处：《实用口腔医学杂志》2024年第6期747-752,共6页Journal of Practical Stomatology

基　　金：国家重点研发计划(编号:2022YFA1104400);国家自然科学基金(编号:82170988)。

摘　　要：目的:解析人第三磨牙牙囊组织的免疫微环境特征,探究先天性免疫细胞和适应性免疫细胞在牙囊组织中的种群组成、相互交流及作用。方法:本研究分析了先前已上传至GSA数据库中的测序数据:GSA-Human:HRA008022,运用生物信息学工具对牙囊组织中的先天性和适应性免疫细胞进行基因鉴定和GO功能富集分析,CellChat分析免疫细胞群之间的相互交流。结果:使用t-SNE降维分析免疫细胞群,分别获得先天性免疫细胞群,包括固有淋巴样细胞、树突状细胞、肥大细胞和巨噬细胞;适应性免疫细胞群包括T细胞和B细胞。Pearson相关性系数分析显示,先天性免疫细胞中固有淋巴样细胞和巨噬细胞与适应性免疫细胞群具有较强的相关性。GO富集分析显示先天性免疫细胞群之间存在相互协同,同时对于适应性免疫细胞群也具有调节作用。进一步的CellChat分析表明先天性免疫细胞群和适应性免疫细胞群之间存在密切的生物学信号传递,其中以CLEC、MIF、ADGRE5、COLLAGEN、MIF信号通路最为显著。结论:牙囊组织中富含大量免疫细胞,其中先天性免疫细胞群和适应性免疫细胞相互作用,共同调节免疫反应,参与维持牙囊组织稳态平衡。Objective:To analyze the immune microenvironment characteristics of human dental follicle tissues from the third molars and to explore the mutual communication and the effects of innate immune cells and adaptive immune cells within the dental follicle.Methods:Sequencing data(GSA-Human:HRA008022)in the GSA database were analyzed.Bioinformatics tools were employed for gene identification and GO enrichment analysis was performed to define the biological function of innate and adaptive immune cells.CellChat analysis was used for explaining intercellular communication among immune cell populations.Results:Using t-SNE dimensionality reduction analysis for immune cell populations,innate immune cell populations were obtained,including innate lymphoid cells,dendritic cells,mast cells and macrophages,and adaptive immune cell populations including T cells and B cells.Pearson correlation analysis showed that innate immune cells,specifically innate lymphoid cells and macrophages,had a strong correlation with adaptive immune cell populations.GO enrichment analysis revealed mutual coordination among innate immune cell populations and regulatory effects on adaptive immune cell populations.Further CellChat analysis indicated biological signal transmission between innate and adaptive immune cell populations,with CLEC,MIF,ADGRE5,COLLAGEN and MIF signaling pathways is the most significant.Conclusion:Dental follicle tissues are rich in immune cells and innate immune cell populations interact with adaptive immune cells to regulate immune responses and participate in maintaining the homeostasis of dental follicle.

关 键 词：单细胞RNA测序 牙囊 免疫 微环境 

分 类 号：R780.2[医药卫生—口腔医学] R329.2[医药卫生—临床医学]