龈下菌斑中微生物与2型糖尿病患者牙周炎的相关性研究  

Correlation analysis of microorganisms in subgingival plaque in patients with T2DM and periodontitis

作　　者：姜明露 范志伟 刘春霞[3] 马向瑞 王文龙 崔彩云 王晶[1] JIANG Minglu;FAN Zhiwei;LIU Chunxia;MA Xiangrui;WANG Wenlong;CUI Caiyun;WANG Jing(Department of Stomatology,Affiliated Hospital of Binzhou Medical University,264003,China;Rizhao Stomatological Hospital;Department of Oral and Maxillofacial Surgery,Affiliated Hospital of Binzhou Medical University)

机构地区：[1]滨州医学院附属医院口腔内科,264003 [2]日照口腔医院 [3]滨州医学院附属医院口腔颌面外科

出　　处：《实用口腔医学杂志》2024年第6期840-848,共9页Journal of Practical Stomatology

基　　金：山东省自然科学基金博士基金(编号:ZR2018BH026);山东省自然科学基金培养基金(编号:ZR2019PH075)。

摘　　要：目的:探讨特殊微生物群落在2型糖尿病(T2DM)患者牙周炎的发生、发展中的作用。方法:纳入20~70岁受试者40人分别为中重度牙周炎伴2型糖尿病组(CP.T2DM,n=15)、中重度牙周炎组(CP,n=15)、健康人组(N,n=10)。记录受试者基本信息、牙周临床指标及血糖等,收集(n=40)龈下菌斑,提取样本DNA,用Illumina NovaSeq6000平台进行基因测序,用Uparse、SPSS等相应软件分析龈下菌斑微生物多样性、生态特征及功能。结果:测序筛选得到共聚类为22个门、30个纲、73个目、129个科、265个属、481个种水平物种。Beta多态性分析显示CP.T2DM组和CP组样本物种组成更相似。3组间Alpha多态性分析显示CP.T2DM组和CP组中的物种丰富度及均匀度显著高于N组(P<0.01)。3组间Venn图分析结果显示CP.T2DM组龈下菌斑的物种丰富程度最高,CP组次之,N组最低。在属水平,Klebsiella和Bifidobacterium在CP.T2DM组中均显著大于CP组和N组(P<0.05),在CP组与N组中无显著性差异。在种水平,CP.T2DM组中的Capnocytophaga leadbetteri显著大于CP组和N组(P<0.05),在CP组与N组中无显著性差异。CP.T2DM、CP及N组间的龈下菌斑微生物群落结构具有一定差异。CP.T2DM组龈下菌群的物种丰富程度高于CP组和N组。结论:中重度牙周炎患者龈下菌群中Klebsiella、Bifidobacterium、Capnocytophaga leadbetter的增加可能与T2DM的发生、发展有关。Objective:To study the role of special microbial communities in the development of periodontitis in type 2 diabetes mellitus(T2DM)patients.Methods:40 subjects aged 20-70 years were included and divided into 3 groups:moderate to severe periodontitis with T2DM(SP.T2DM,n=15),moderate to severe periodontitis group(SP,n=15)and normal healthy group(N,n=10).The basic information,periodontal clinical indicators and blood sugar of the subjects were recorded.Subgingival plaque samples were collected,DNA samples of the plaque were extracted,and sequenced by Illumina NovaSeq6000 platform.The microbial diversity,ecological characteristics and functions of the plaque were analyzed by Uparse,SPSS and other softwares.Results:481 species in 22 phyla,30 classes,73 orders,129 families and 265 genera were obtained from the samples.Beta polymorphism analysis showed that the species composition of CP.T2DM group and CP group was similar.Alpha polymorphism analysis showed that the species richness and evenness in CP.T2DM group and CP group were higher than those in N group(P<0.01).Venn diagram analysis showed that the species richness of the plaque in CP.T2DM group was the highest,followed by CP group and the lowest in N group.At the genus level,Klebsiella and Bifidobacterium in CP.T2DM group were larger than those in CP group and N group(P<0.05),and between group CP and N,P>0.05.At the species level,the Capnocytophaga leadbetteri in CP.T2DM group was higher than that in CP group and N group(P<0.05),between group CP and N,P>0.05;There were some differences in the microbial community structure of subgingival plaque among the 3 groups.The species richness of subgingival flora in patients with CP and T2DM was higher than that in patients with CP and healthy people.Conclusion:The increase of Klebsiella,Bifidobacterium and Capnocytophaga leadbetter in subgingival flora of patients with moderate and severe periodontitis may be related to the development of T2DM.

关 键 词：中重度牙周炎 2型糖尿病 龈下菌斑 微生物群落 16SrDNA测序 

分 类 号：R780.2[医药卫生—口腔医学]