莓叶委陵菜叶绿体基因组特征及其系统发育分析  

Chloroplast Genome Characteristics and Phylogenetic analysis of Potentilla fragarioides

作　　者：郭松 梁湘兰 彭姿 卢全 曾珍贵 GUO Song;LIANG Xiang-lan;PENG Zi;LU Quan;ZENG Zhen-gui(Smart Agriculture College(Internet of Things Engineering College)of Guangxi Science&Technology Normal University,Laibin,Guangxi Province 546199,China;Key Laboratory for Zhuang and Yao Pharmaceutical Quality Biology,Guangxi Science&Technology Normal University,Laibin,Guangxi Province 546199,China;College of Biology and Food Engnineering of Guangxi Science&Technology University,Laibin,Guangxi Province 546199,China)

机构地区：[1]广西科技师范学院智慧农业学院(物联网工程学院),广西来宾546199 [2]广西科技师范学院壮瑶药品质生物学重点实验室,广西来宾546199 [3]广西科技师范学院生物与食品工程学院,广西来宾546199

出　　处：《草地学报》2024年第11期3383-3390,共8页Acta Agrestia Sinica

基　　金：广西高校中青年教师科研基础能力提升项目(2021KY0862);广西科技师范学院壮瑶药品质生物学重点实验室经费(GXKSKYPT2021007);广西科技基地与人才专项(2021AC19423)资助。

摘　　要：为解析莓叶委陵菜叶绿体基因组特征,探究其在蔷薇科物种中的发育关系,本研究通过莓叶委陵菜叶绿体基因组的测序、组装、注释以及结合生物信息方法获得其叶绿体全基因组特征信息,并构建了系统发育进化树。结果表明,莓叶委陵菜叶绿体基因组全长156844 bp,为一个典型的四分体结构,GC含量GC_(3)<GC_(2)<GC_(1),密码子偏好以A/T结尾,IR边界分析表明,其基因组结构较为保守。通过与蔷薇科下草莓属、蔷薇属、委陵菜属的13个物种进行系统发育分析,得到莓叶委陵菜与蛇莓的亲缘关系较近,以100%自举值聚到一起。本研究将有助于莓叶委陵菜的系统发育和遗传研究。In order to determine the chloroplast genome characteristics of Potentilla fragarioides and to investigate its phylogenetic relationship within Rosaceae species,this study performed sequencing,assembly,and annotation of the chloroplast DNA genome of P.fragarioides,and obtained comprehensive information about its chloroplast genome by integrating bioinformatics methods.Based on this,a phylogenetic tree was constructed.The results showed that P.fragarioides complete chloroplast genome is 156844 bp,which has a typical quadripartite structure with GC content of GC_(3)<GC_(2)<GC_(1),and a codon usage bias with A/T ending.The analysis of the IR boundary indicated that the genome structure of P.fragarioides is relatively conservative.Through phylogenetic analysis of 13 species in the Rosaceae family,including Fragaria,Rosa,and Potentilla,P.fragarioides was found to be closely related to Potentilla indica,with a bootstrap value of 100%.This study will contribute to the systematic and genetic research of P.fragarioides.

关 键 词：莓叶委陵菜 叶绿体基因组 系统发育 瑶药 

分 类 号：Q789[生物学—分子生物学]