SOX4靶向miR-17对结肠癌细胞HCT-116生物学行为的影响  

SOX4 and miR-17 in colorectal cancer and their effects on HCT-116 cells

作　　者：张纲 刘楠 王国强 王伟 武雪亮[3] 郑海平 张玉国 ZHANG Gang;LIU Nan;WANG Guo-qiang;WANG Wei;WU Xue-liang;ZHENG Hai-ping;ZHANG Yu-guo(Department of General Surgery,the First Hospital of Zhangjiakou City,Zhangjiakou 075000,China;Department of Pathology,the First Hospital of Zhangjiakou City,Zhangjiakou 075000,China;Department of General Surgery,the First Affiliated Hospital of Hebei North University,Zhangjiakou 075000,China)

机构地区：[1]张家口市第一医院普通外科,河北张家口075000 [2]张家口市第一医院病理科,河北张家口075000 [3]河北北方学院附属第一医院普通外科,河北张家口075000

出　　处：《诊断病理学杂志》2024年第11期1072-1078,共7页Chinese Journal of Diagnostic Pathology

基　　金：河北省医学科学研究课题计划(20211329)。

摘　　要：目的探讨性别决定区Y框蛋白4(SOX4)及miR-17在结直肠癌中的表达及对HCT-116细胞生物学行为的作用。方法收集2020年3月至2022年1月在河北北方学院一附院和张家口市第一医院的27例结直肠癌患者组织标本并检测SOX4及miR-17的表达水平,并分析与临床病理特征的关系。利用慢病毒转染技术干预HCT-116细胞的SOX4及miR-17表达。检测各组细胞中SOX4和miR-17的表达及细胞增殖、侵袭、迁移能力、细胞活性变化和相关蛋白表达。结果与正常组织比较,结直肠癌组织中SOX4表达水平升高。SOX4及miR-17在结直肠癌组织中的表达呈正相关(r=0.724,P<0.001),SOX4和miR-17的表达和病理分期、淋巴结转移、肝转移情况相关,差异有统计学意义(P<0.05),而与肿瘤的大小、分化程度无相关(P>0.05)。SOX4模拟组细胞增殖能力、迁移能力及细胞活性显著高于对照组及SOX4抑制剂组(P<0.05)。SOX4抑制剂组与对照组相比迁移及增殖相关蛋白表达差异有统计学意义(P<0.05)。结论SOX4在结直肠癌组织中表达上调,SOX4的表达可促进结肠癌细胞增殖、迁移和侵袭能力,miR-17可能是其下游调控靶点。Objective To investigate the expression of sex-determining region Y-box 4(SOX4)and miR-17 in colorectal cancer and their effects on the biological behavior of HCT-116 cells.Methods The tissue samples were collected from 27 colorectal cancer patients at Hebei North University First Affiliated Hospital and Zhangjiakou First Hospital from March 2020 to January 2022.The expression levels of SOX4 and miR-17 were detected with immunohistochemistry,and their relationship with clinical pathological characteristics was analyzed.Intervention of SOX4 and miR-17 expression in HCT-116 cells was determined with lentiviral transfection technology.The expression of SOX4 and miR-17 was also detected in each group of cells,as well as changes in cell proliferation,invasion,migration ability,cell activity,and related protein expression were evaluated.Results Compared with normal tissues,the expression level of SOX4 was elevated in colorectal cancer tissues.The expression of SOX4 and miR-17 in colorectal cancer tissues was positively correlated(r=0.724,P<0.001).The expression of SOX4 and miR-17 was statistically significant difference with the pathological stage,lymph node metastasis,and liver metastasis(P<0.05),but not with tumor size and differentiation degree(P>0.05).The proliferation ability,migration ability,and cell activity of SOX4 overexpressing cells were significantly higher than those of the control group and SOX4 expression inhibition group(P<0.05).There was a statistically significant difference in the expression of migration and proliferation related proteins between the SOX4 expression inhibition group and the control group(P<0.05).Conclusions SOX4 is upregulated in colorectal cancer tissues,promoting proliferation,migration,and invasion of colon cancer cells.miR-17 may be a downstream regulatory target of SOX4.

关 键 词：结直肠癌 性别决定区Y框蛋白4 miR-17基因簇 人结肠癌HCT-116细胞 增殖 侵袭 

分 类 号：R735.7[医药卫生—肿瘤]