Anti-melanogenic properties of FBCC-EP850 derived from Carex pumila Thunb  

作　　者：Mirissa Hewage Dumindu Kavinda Jinkuk Park Nayeong Kim Yung Hyun Choi Gi-Young Kim 

机构地区：[1]Department of Marine Life Science,Jeju National University,Jeju 63243,Republic of Korea [2]Department of Biochemistry,College of Korean Medicine,Dong-Eui University,Busan 47227,Republic of Korea

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2024年第11期477-485,共9页亚太热带生物医学杂志（英文版）

基　　金：supported by the Korea Environmental Industry&Technology Institute through Project to Make Multi-ministerial National Biological Research Resources more Advanced funded by Korea Ministry of Environment(RS-2021-KE001783).

摘　　要：Objective:To elucidate the anti-melanogenic potential of Carex pumila Thunb.extract(FBCC-EP850).Methods:A collection of 180 plant extracts was tested for inhibition of mushroom tyrosinase activity using an in vitro assay.Among them,FBCC-EP850 exhibited the most promising inhibitory activity.Further analysis was conducted to investigate its mechanisms and therapeutic potential in reducing melanogenesis in B16F10 melanoma cells and zebrafish larvae.Results:FBCC-EP850 inhibited mushroom tyrosinase activity in a dose-dependent manner,with a half-maximal inhibitory concentration of 45.83μg/mL.FBCC-EP850 at concentrations up to 50μg/mL demonstrated minimal cytotoxicity against B16F10 melanoma cells and no adverse effects on zebrafish larvae.Treatment with 50μg/mL of FBCC-EP850 significantly reducedα-melanocyte stimulating hormone-induced melanin production and suppressed cellular tyrosinase activity in B16F10 melanoma cells.Additionally,FBCC-EP850 at 25 and 50μg/mL effectively diminished hyperpigmentation inα-melanocyte stimulating hormone-stimulated zebrafish larvae.Its anti-melanogenic action could be attributed to modulation of the cAMP-CREB-MITF signaling pathway.Conclusions:Carex pumila extract can inhibit melanogenesis by modulating the cAMP-CREB-MITF signaling pathway,which can be used as a promising candidate for treating hyperpigmentation disorders.

关 键 词：Carex pumila Thunb. MELANOGENESIS TYROSINASE cAMP-CREB-MITF signaling 

分 类 号：R285.5[医药卫生—中药学]