对乙酰氨基酚致大鼠急重症药物性肝损伤的细胞因子变化研究  

Study on the changes of cytokines in rats with acute and severe drug-induced liver injury induced by acetaminophen

作　　者：何玲玲[1] 黄庭龙[1] 谢龙[2] HE Lingling;HUANG Tinglong;XIE Long(Department of Critical Care Medicine;Department of Nuclear Medicine,the Second Affiliated Hospital of Fujian Medical University,Quanzhou,Fujian 362000,China)

机构地区：[1]福建医科大学附属第二医院重症医学科,福建泉州362000 [2]福建医科大学附属第二医院核医学科,福建泉州362000

出　　处：《现代医药卫生》2024年第22期3786-3790,共5页Journal of Modern Medicine & Health

基　　金：福建省自然科学基金项目(2021J01277)。

摘　　要：目的了解大鼠模型中对乙酰氨基酚致肝急性损伤的细胞因子变化,为临床提供诊疗思路。方法将12只雄性SD大鼠,平均分为空白组和对乙酰氨基酚组(实验组)各6只。实验组每只大鼠用750 mg/kg对乙酰氨基酚腹腔注射,12 h后可造模成功,观察大鼠肝组织损伤程度;空白组不作任何处理。检测2组血清白细胞介素-6(IL-6)水平及肝组织谷草转氨酶(AST)/谷丙转氨酶(ALT)的表达及肿瘤坏死因子-α(TNF-α)、趋化因子(CXCL)-10的mRNA表达水平。结果实验组大鼠肝脏损伤评分[(5.3±0.8)分]高于空白组(0分),差异有统计学意义(P<0.01)。实验组大鼠肝脏组织AST、ALT水平[分别为(107.6±16.7)、(35.1±5.2)U/g]低于空白组[分别为(178.8±21.6)、(58.9±6.8)U/g],差异均有统计学意义(P<0.01)。实验组大鼠血清中IL-6的表达水平[(67.9±4.8)pg/mL]明显高于空白组[(4.8±0.4)pg/mL],差异有统计学意义(P<0.01)。实验组大鼠肝脏组织中TNF-α、CXCL-10表达水平[分别为(3.34±0.50)、(4.15±0.54)]均高于空白组[分别为(1.00±0.14)、(1.00±0.15)],差异均有统计学意义(P<0.01)。结论对乙酰氨基酚可造成大鼠药物性肝损伤,检测血清IL-6水平、肝组织中AST、ALT的表达及TNF-α、CXCL-10的mRNA表达水平变化有助于疾病的诊断。Objective To understand the changes of cytokines in acetaminophen-induced acute liver injury in rat models,and to provide ideas for clinical diagnosis and treatment.Methods A total of 12 male SD rats were equally divided into blank group and acetaminophen group(the experimental group),with six rats in each group.Each rat of experimental group was intraperitoneally injected with 750 mg/kg acetaminophen.After 12 hours,the model was successfully established.The degree of liver tissue injury of rats was observed.The blank group with nothing.The levels of serum interleukin-6(IL-6),the expression of aspartate aminotransferase(AST)/alanine aminotransferase(ALT),and the mRNA expression levels of TNF-αand CXCL-10(in two groups)were detected.Results The liver injury score of rats in the experimental group[(5.3±0.8)scores]was higher than that in the blank group(0 score),and the difference was statistically significant(P<0.01).The levels of AST and ALT in liver tissue of rats in the experimental group[(107.6±16.7),(35.1±5.2)U/g]were lower than those in the blank group[(178.8±21.6),(58.9±6.8)U/g],and the differences were statistically significant(P<0.01).The expression level of IL-6 in serum of rats in the experimental group[(67.9±4.8)pg/mL]was significantly higher than that in the blank group[(4.8±0.4)pg/mL],and the difference was statistically significant(P<0.01).The expression levels of TNF-αand CXCL-10 in liver tissue of rats in the experimental group[(3.34±0.50)and(4.15±0.54),respectively]were higher than those in the blank group[(1.00±0.14)and(1.00±0.15),respectively],and the differences were statistically significant(P<0.01).Conclusion Acetaminophen can cause drug-induced liver injury in rats.Detectting the changes of serum IL-6 level,the expression of AST and ALT in liver tissue and the mRNA expression of TNF-αand CXCL-10 are helpful for the diagnosis of the disease.

关 键 词：急重症 对乙酰氨基酚 药物性肝损伤 细胞因子 大鼠 

分 类 号：R575.3[医药卫生—消化系统]