育成期代谢能摄入量对蛋鸡生殖器官发育、激素水平和卵巢基因表达的影响  

Effects of Metabolizable Energy Intake during Rearing on Development of Reproductive Organs,Hormone Level and Gene Expression in Ovary of Laying Hens

作　　者：卢建[1] 居小军 王星果[1] 马猛[1] 王强[1] 李永峰 窦套存[1] 胡玉萍[1] 郭军[1] 邵丹[1] 童海兵[1] 曲亮[1] LU Jian;JU Xiaojun;WANG Xingguo;MA Meng;WANG Qiang;LI Yongfeng;DOU Taocun;HU Yuping;GUO Jun;SHAO Dan;TONG Haibing;QU Liang(Jiangsu Institute of Poultry Sciences,Yangzhou 225125,China)

机构地区：[1]江苏省家禽科学研究所,扬州225125

出　　处：《畜牧兽医学报》2024年第11期5085-5100,共16页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：江苏省种业振兴揭榜挂帅项目(JBGS[2021]104);现代农业产业技术体系建设专项资金(CARS-40-K01);江苏现代农业产业技术体系建设项目(JATS[2023]069)。

摘　　要：旨在研究育成期代谢能(ME)摄入量对蛋鸡生殖器官发育、激素水平和卵巢基因表达的影响。将720只6周龄海兰褐蛋鸡随机分为3组,每组6个重复,每个重复40只鸡。6~17周龄,各试验组饲粮粗蛋白质水平分别为17.5%(6~12周龄)和15.5%(12~17周龄),ME水平分别为12.34、11.11(12.34×90%)和9.87(12.34×80%)MJ·kg-1(分别为对照组亦即自由采食组、90%ME限饲组和80%ME限饲组),其他营养素水平相同。对照组试验鸡自由采食,其他试验组蛋鸡按照对照组蛋鸡采食量定量饲喂。试验期为6~17周龄。结果表明:1)随着育成期能量限饲强度增加,各试验组蛋鸡17周龄体重、体斜长和跖围均显著线性减少(P<0.001)。2)随着育成期能量限饲强度增加,血清尿素氮(UN)和葡萄糖(GLU)水平均显著线性减小(P=0.040,P=0.044),但血浆雌二醇水平显著线性增加(P=0.026)。3)对17周龄血液雌二醇水平差异最显著的自由采食组(ALF17W)和80%能量限饲组(ERF17W)蛋鸡卵巢基质部进行RNA-Seq分析,纯净序列匹配到鸡参考基因组的比例均超过了94.61%,Q20和Q30的纯净序列含量分别高于97.38%和92.87%,两组共筛选出1299个差异基因,ERF17W中961个下调,338个上调。GO功能富集分析发现,差异表达的mRNAs参与调节细胞增殖、发育和生殖等48个显著富集的GO条目,KEGG信号通路显著富集在25个显著富集的KEGG通路,其中cAMP信号通路、雌激素信号通路、类固醇激素生物合成和卵巢类固醇生成等是与能量代谢或生殖相关的通路。筛选到的cAMP反应元件结合蛋白(CREB)和类固醇生成急性调节蛋白(StAR)富集在cAMP信号通路,孕激素受体(PGR)、代谢型谷氨酸受体1(GRM 1)和细胞骨架蛋白角蛋白18(KRT 18)富集在雌激素信号通路。qRT-PCR结果显示10个随机选择的差异表达基因的表达趋势与RNA-Seq结果一致。综上可见,随育成期ME摄入量减少,育成期末蛋鸡体重、血清UN和GLU含量均显著线性减小,但�This experiment was conducted to evaluate the effects of metabolizable energy(ME)intake during rearing on the growth and development of reproductive organ,hormone level and gene expression in ovary of laying hens.A total of 7206-week-old Hyline-Brown laying hens were allocated equally to three groups with six replicates of 40 hens each,and were fed one of three diets that were nutritionally equal with the exception of ME levels.From 6 to 17 weeks of age,the crude protein(CP)levels in diet were 17.5%(6-12 weeks)and 15.5%(12-17 weeks),respectively,and the ME levels in diet were 12.34,11.11(12.34×90%)and 9.87(12.34×80%)MJ·kg-1(control group,also called ad libitum feeding group,90%energy-restricted feeding group,and 80%energy-restricted feeding group),respectively.The hens in control group were fed ad libitum,and the daily amount of feed in experimental groups was restricted to the absolute quantity of the diet consumed by hens in control group.The results showed as follows:1)Body weight,body slope length and shank circumference of hens at 17 weeks of age decreased linearly(P<0.001)with increasing levels of energy restriction.2)The serum UN and GLU content decreased linearly(P=0.040 and 0.044,respectively),while the plasma oestradiol concentrations increased linearly(P=0.026).3)The ovary stroma of hens in the ad libitum feeding group(ALF17W)and 80%energy-restricted feeding group(ERF17W)with significant differences(P<0.05)in plasma oestradiol concentrations were used to screen the novel mRNA implemented by the RNA-seq.The proportion of pure reads matching to chicken reference genome was more than 94.61%,and the content of Q20 and Q30 was more than 97.38%and 92.87%,respectively.A total of 1299 differential genes were screened in ALF17W and ERF17W,of which 961 were down-regulated and 338 were up-regulated in ERF17W.The GO functional enrichment analysis found that differentially expressed mRNAs were involved in 48 GO terms such as cell proliferation,development,and reproduction.The KEGG pathway were significantly enri

关 键 词：RNA-SEQ 育成期 代谢能摄入量 雌激素 卵巢 蛋鸡 

分 类 号：S831.5[农业科学—畜牧学]